Earticle

초록

영어

Ethanol productions were performed by separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) processes using seaweed, Enteromorpha intestinalis (sea lettuce). Pretreatment conditions were optimized by the performing thermal acid hydrolysis and enzymatic hydrolysis for the increase of ethanol yield. The pretreatment by thermal acid hydrolysis was carried out with different sulfuric acid concentrations in the range of 25 mM to 75 mM H2SO4, pretreatment time from 30 to 90 minutes and solid contents of seaweed powder in the range of 10~ 16% (w/v). Optimal pretreatment conditions were determined as 75 mM H2SO4 and 13% (w/v) slurry at 121oC for 60 min. For the further saccharification, enzymatic hydrolysis was performed by the addition of commercial enzymes, Celluclast 1.5 L and Viscozyme L, after the neutralization. A maximum reducing sugar concentration of 40.4 g/L was obtained with 73% of theoretical yield from total carbohydrate. The ethanol concentration of 8.6 g/L of SHF process and 7.6 g/L of SSF process were obtained by the yeast, Saccharomyces cerevisiae KCTC 1126, with the inoculation cell density of 0.2 g dcw/L.

목차

Abstract
 1. Introduction
 2. Materials and Methods
  2.1. Raw materials and characterization
  2.2. Thermal acid hydrolysis
  2.3. Enzymatic hydrolysis of pretreated seaweed slurry
  2.4. Yeast cultivation
  2.5. Ethanol fermentation
  2.6. Analysis of reducing sugar, monosaccharide and viscosity
 3. Results and Discussions
  3.1. Composition and characterization of E. intestinalis
  3.2. Effect of pretreatment condition
  3.3. Hydrolysis of E. intestinalis by commercial enzymes
  3.4. SSF and SHF
 4. Conclusions
 ACKNOWLEDGEMENTS
 REFERENCES

키워드

저자

• YuKyeong Cho [ Department of Biotechnology, Pukyong National University, Busan ]
• Min-Ji Kim [ Department of Biotechnology, Pukyong National University, Busan ]
• Sung-Koo Kim [ Department of Biotechnology, Pukyong National University, Busan ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576