Earticle

초록

영어

A polysaccharide degrading enzyme, strain SC092 was isolated from the seawater. This strain was identified as Microbulbifer sp. using the comparative sequence analysis against known 16S rRNA sequence. A polysaccharide degrading enzyme from this strain was used to acquire the enzymatic extracts of Sargassum fulvellum. DPPH radical scavenging and SOD activity of the enzyme extracts of S. fulvellum were about 61.9% and 82.9% at 2 mg/mL, respectively. Nitrite scavenging activities was 52.5% at 2 mg/mL on pH 1.2. In addition, α-glucosidase inhibitory activity was also increased in a dose-dependent manner and was about 52.7% at 2 mg/mL. To determine the influence of enzyme extracts of S. fulvellum on alcohol metabolism, the generating activity of reducednicotinamide adenine dinucleotide (NADH) by alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) were measured. ADH and ALDH activities were 118.0% and 177% at 2 mg/mL, respectively. α-Glucosidase inhibitory activity of enzyme extracts of S. fulvellum was remarkably increased in a dose-dependent manner and was about 52.7% at 2 mg/mL. These results indicate alcoholizing and α-Glucosidase inhibitory activities can be enhanced by the enzymatic extracts of S. fulvellum.

목차

Abstract
 1. 서론
 2. 재료 및 방법
  2.1. 시료조제
  2.2. 균주 및 조효소 조제
  2.3. 조효소활성 측정
  2.4. 참모자반 효소분해산물 제조
  2.5. DPPH radical 소거능 측정
  2.6. Superoxide dismutase (SOD) 활성 측정
  2.7. 아질산염 소거능 측정
  2.8. Alcohol dehydrogenase (ADH) 활성 측정
  2.9. Acetaldehyde dehydrogenase (ALDH) 활성측정
  2.10. α-Glucosidase 활성억제 효과 측정
 3. 결과 및 고찰
  3.1. 참모자반 효소분해 산물
  3.2. DPPH assay
  3.3. Superoxide dismutase (SOD) 활성 측정
  3.4. 아질산염 소거능 측정
  3.5. 알코올 분해 활성 (ADH, ALDH) 측정
  3.6. α-Glucosidase 활성억제 효과
 4. 결론
 참고문헌

키워드

저자

• 조은경 [ Eun Kyung Cho | 신라대학교 식품영양학과 ]
• 강수희 [ Su Hee Kang | 신라대학교 식품영양학과 ]
• 최영주 [ Young Ju Choi | 신라대학교 식품영양학과 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576