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Statistical Optimization of Media Components for Enhanced Production of the Recombinant Staphylokinase Variant from Salt Inducible E.Coli GJ1158
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- 발행기관
- 보안공학연구지원센터(IJBSBT) 바로가기
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- 간행물
- International Journal of Bio-Science and Bio-Technology SCOPUS 바로가기
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- 통권
- Vol.4 No.4 (2012.12)바로가기
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- 페이지
- pp.27-40
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A207093
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
- 영어
- Different types of nutritional factors are influencing the production of recombinant staphylokinase variant from Msak - Hirulog pRSET-A GJ1158, which was constructed in our lab. The optimal conditions for the production of recombinant staphylokinase variant by using submerged fermentation at 370C for 6 hours after induction with 300 mM NaCl at flask level with a working volume of 100 ml. Initially, all nutritional factors were selected by one factor- at-a-time method. The significance of each factor with respect to recombinant staphylokinase production was identified by Taguchi (7 factors viz.,glucose, K2HPO4, TMM, KH2PO4, NH4Cl, yeast extract and MgSO4)and the enzymatic activity was increased remarkably by 1.32 folds compared to the test tube level (7580 to 10,081 U/mL). Based on taguchi results glucose, K2HPO4, TMM and KH2PO4 are the most influencing parameters. The outcome of taguchi design showed that the further optimization using response surface methodology (Central Composite Design) with 30 experiments increased the yield of 1.98 folds (10,081to 19,928 U/ml). For the first time we optimized the components concentration for enhanced production of sak variants and having maximum activity.
목차
Abstract
1. Introduction
2. Materials and Methods
2.1 Medium Components
2.2 Media Preparation and Culture Conditions
2.3 Optimization by using one Factor-at-a-time
2.4 Optimization of Media Components by using L8 - Orthogonal Array
2.5 Estimation of r-SAK Assay
2.6 Optimization of Components of the Selected Medium by RSM
3. Results & Discussion
3.1 One Factor-at-a-time Method
3.2 Optimization using L8-orthogonal Array
3.3 Optimization of Concentrations of the Selected Medium Components by RSM
4. Conclusion
Acknowledgements
References
키워드
recombinant staphylokinase one factor–at–a–time method Taguchi central composite design enzyme activity저자
간행물 정보
발행기관
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- 발행기관명
- 보안공학연구지원센터(IJBSBT) [Science & Engineering Research Support Center, Republic of Korea(IJBSBT)]
- 설립연도
- 2006
- 분야
- 공학>컴퓨터학
- 소개
- 1. 보안공학에 대한 각종 조사 및 연구 2. 보안공학에 대한 응용기술 연구 및 발표 3. 보안공학에 관한 각종 학술 발표회 및 전시회 개최 4. 보안공학 기술의 상호 협조 및 정보교환 5. 보안공학에 관한 표준화 사업 및 규격의 제정 6. 보안공학에 관한 산학연 협동의 증진 7. 국제적 학술 교류 및 기술 협력 8. 보안공학에 관한 논문지 발간 9. 기타 본 회 목적 달성에 필요한 사업
간행물
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- 간행물명
- International Journal of Bio-Science and Bio-Technology
- 간기
- 격월간
- pISSN
- 2233-7849
- 수록기간
- 2009~2016
- 등재여부
- SCOPUS
- 십진분류
- KDC 505 DDC 605
이 권호 내 다른 논문 / International Journal of Bio-Science and Bio-Technology Vol.4 No.4
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