Earticle

초록

영어

Every aspect of tumor growth required vascular growth. Many natural products inhibited angiogenesis. The makabuhay plant, Tinospora rumphii Boerl was an important species of Menispermaceae family with common utilization in folk medicine in the Philippines. This study aimed to evaluate the angiosuppressive activity of Tinospora rumphii Boerl. stem extract using the chorioallantoic membrane assay in duck’s (Anas platyrhynchos) embryos. There were thirty pieces of one day old fertile duck eggs used in the study. The setups were composed of a positive control (Retinoic acid), negative control (95% ethanol) and makabuhay stem extracts in 25%, 50%, 75% and 100% concentrations. The different concentrations of the extracts were applied on the tenth day of incubation. The antiangiogenic effect of T. rumphii Boerl stem extract was evaluated by taking the average number of branch points using the chorioallantoic membrane. This was determined on the 12th day of incubation. One-way analysis of variance and Scheffe test for significant antiangiogenic activity and pair-wise comparison among the treatments at 0.05 alpha level were determined respectively. Results showed that angiogenesis was induced in the negative control while angiogenesis inhibition was significantly reduced in the treatment of the stem extracts. It was supported by the statistical analysis that there was a significant difference on the antiangiogenic effect of makabuhay stem extract using the CAM assay on the vascularization of duck embryos. A significant difference was indicated between the negative control and among the treatments used. It showed that the greater the dosage, the lesser the branch points observed and counted. Pure makabuhay stem extract had the lowest branch points mean value while the 25% concentration had the highest mean value among the treatments used. Thus, these findings of the study indicated that T. rumphii Boerl. stem extract might had a promising antiangiogenic potential.

목차

Abstract
 1. Introduction
  1.1 Background of the Study
  1.2 Statement of the Problem
  1.3 General Objective
  1.4 Specific Objectives
  1.5 Significance of the Study
 2. Methodology
  2.1 Plant Material
  2.2. Filter Paper Disc Preparation
  2.3. Extract Preparation
  2.4 Assay Proper
  2.5 Sampling Treatment in a Completely Randomized Design (CRD).
  2.6 Visual Assessment and Photography.
  2.7 Statistical Analysis.
 3. Results and Discussion
  3.1. Results
  3.2 Statistical Data Analysis
  3.3 Discussion
 4. Conclusions and Recommendations
 Acknowledgments
 References

키워드

저자

• Rey G. Tantiado [ College of Arts and Sciences, Iloilo Doctors’ College, West Avenue, Molo, Iloilo City and West Visayas State University, La Paz, Iloilo City, Philippines ]
• Virgie P. Tan [ West Visayas State University Extension Campus Himamaylan City, Negros Occidental, Philippines ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  International Journal of Bio-Science and Bio-Technology

• 간기

  격월간

• pISSN

  2233-7849

• 수록기간

  2009~2016

• 등재여부

  SCOPUS

• 십진분류

  KDC 505 DDC 605