Biolog Ecoplate와 DGGE 방법을 이용한 알칼리화 토양의 미생물군집 변화 평가
Assessment of the Changes in the Microbial Community in Alkaline Soils using Biolog Ecoplate and DGGE
Soil microbial community analysis of farmland soil sprayed with lye in order to use fertilizer in Nigeria was performed. As a control, two kinds of soils not sprayed with lye, located in Eungo and Lagos with general practice in agriculture was selected. Soil sprayed with lye was pH 8.25 through alkalization reaction, while the other soil samples were pH 6.22 and 5.94. Substrate utilization and species diversity index of soil sprayed with lye were low than that of the other soils with the analysis of Biolog ecoplate. As a result of principal component analysis, the relationship between three samples was low. Microbial community analysis was performed by DGGE and most of them were soil uncultured bacterium. Especially, Uncultured Acidobacteria and Uncultured Methylocystis sp., which had been isolated from the rhizosphere of soybean grown in that site were discovered in the soil sprayed with lye.
목차
Abstract 1. 서론 2. 실험재료 및 방법 2.1. 토양의 시료채취와 pH 측정 2.2. Community-level physiological profiling (CLPP) 분석 2.3. Denaturing gradient gel electrophoresis (DGGE) 기법을 이용한 미생물 군집 분석 3. 결과 및 고찰 3.1. 토양의 pH 3.2. Community-level physiological profiling (CLPP) 분석 3.3. Denaturing gradient gel electrophoresis (DGGE) 기법을 이용한 미생물 군집 분석 4. 결론 사사 REFERENCES
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동