Genomic DNAs of psychrophilic strains of Pleurotus eryngii were analyzed by randomly amplified polymorphic DNA (RAPD) using OP-A, OP-B, OP-L, OP-P, OP-R and OP-S3 primers to develop the strain-specific DNA marker. A unique DNA fragment with the size of 480 bp was yielded by OP-S3 primer from the psychrophilic strain. A sequence characterized amplified region (SCAR) marker, designated as OP-S3-1, was designed on the basis of the determined sequence. The PCR analysis with the OP-S3-1 primer showed that this SCAR marker can clearly distinguish the psychrophilic strains from the control strains.
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ABSTRACT 공시균주 및 저온적응성 계통 선발 마커 탐색을 위한 RAPD 저온성 계통에 특이적인 PCR 산물의 Cloning 및 염기서열 분석 SCAR marker 디자인 적요 감사의 글 참고문헌