Pholiota species were collected from different geographical regions of the world. Genetic diversity and phylogenetic relationships were analyzed by rDNA-ITS sequences and RAPD polymorphism. The sizes of rDNA-ITS PCR amplicons of Pholiota spp. varied from 233~271, 158~223 and 174~219 bp, respectively. A phylogenetic tree was constructed on the ITS region sequences and Pholiota strains were classified into 8 clusters. Twenty strains in seven Pholiota spp. were classified into seven clusters by RAPD polymorphism using 15 arbitrary primers. Our experimental results suggested that rDN-ITS and RAPD analysis are useful tool for classifying Pholiota spp. and strains.
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ABSTRACT 서론 재료 및 방법 실험 버섯 균주 버섯균사체 배양 DNA의 추출 rDNA ITS영역의 PCR 증폭 DNA 염기서열 결정 DNA 염기서열 및 유연관계 분석 RAPD 분석 결과 및 고찰 ITS 영역의 염기서열을 이용한 유연관계 분석 RAPD를 이용한 계통분석 적요 감사의 글 참고문헌