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소 정자의 동결 및 수정능력 향상을 위한 정액운반법의 개발
Development of Semen Transport System for Cryopreservation and Fertility in Bull Sperm

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 37 No 3 (2013.09)바로가기
  • 페이지
    pp.97-102
  • 저자
    이상희, 송은지, 우제석, 이승환, 강희설, 정희태, 양부근, 박춘근
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A204126

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원문정보

초록

영어
The objective of this study was to develop of semen transport system for cryopreservation and fertility in bull sperm. The ejaculated semen were diluted with Triladyl containing 20% egg-yolk for transportation. Diluted semen was transported by three methods that there were wrapping tissue (Tissue), sinking under 30℃ water (Water) and sinking between warm water and air (Air) methods. Semen was transported within 2 hours in 0.3℃. For this study, the freezing of diluted semen were added with Triladyl containing 20% egg-yolk. And frozen-thawed sperm were estimated with SYBR14/PI double stain for viability, FITC-PNA/PI double stain for acrosome reaction analysis and Rhodamine123 double stain for mitochondrial intact assessment. In results, live sperm (SYBR+/PI-) in Air treatment group (43.3±4.7%) was significantly (p<0.05) higher than other treatment groups (Tissue: 16.3±2.7% and Water: 27.5± 3.1%), dying sperm (SYBR+/PI+) in Air treatment group (55.6±4.7%) was significantly lower than other treatment groups (Tissue: 77.6±3.2% and Water: 67.6±3.3%) (p<0.05). Acrosome reaction in Air treatment group (0.2±0.1%) within live sperm (PI negative region) was significantly (p<0.05) lower than other treatment groups (Tissue: 0.7±0.2% and Water: 0.5±0.1%), the acrosome reaction in Air treatment group (28.6±2.8%) within all sperm also was significantly lower than other treatment groups (Tissue: 44.2±1.8% and Water: 36.2±2.0%) (p<0.05). And mitochondrial intact in Air treatment group within live (97.1±0.4%) and all (61.9±3.3%) sperm were significantly higher than other treatment groups (Tissue: 85.2±3.3%, Water: 87.8±2.9% within live sperm and Tissue: 49.28±3.7%, Water: 42.0±3.1% within all sperm) (p<0.05). Therefore, we suggest that transportation by sinking method between warm water and air was beneficial to improvement of fertility in frozen-thawed in bull semen.

목차

ABSTRACT
 서론
 재료 및 방법
 결과
 고찰
 감사의 글
 인용문헌

키워드

Bull sperm Cryopreservation Transport system Flow cytometry

저자

  • 이상희 [ Sang-Hee Lee | 강원대학교 동물생명과학대학 ]
  • 송은지 [ Eun-Ji Song | 강원대학교 동물생명과학대학 ]
  • 우제석 [ Jea-Seok Woo | 국립축산과학원 한우시험장 ]
  • 이승환 [ Seung-Hwan Lee | 국립축산과학원 한우시험장 ]
  • 강희설 [ Hee-Seol Kang | 국립축산과학원 한우시험장 ]
  • 정희태 [ Hee-Tae Cheong | 강원대학교 수의학과 ]
  • 양부근 [ Boo-Keun Yang | 강원대학교 동물생명과학대학 ]
  • 박춘근 [ Choon-Keun Park | 강원대학교 동물생명과학대학 ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 37 No 3

    피인용수 : 0(자료제공 : 네이버학술정보)

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