Earticle

초록

영어

Biochemical characteristics of 24 Pongamia pinnata genotypes (candidate plus trees) from three agroclimatic zones were estimated and molecular characterization through RAPD markers was done. Various biochemical characters viz. seed oil, total carbohydrates, protein, acid value and Iodine number recorded significant variation among different genotypes. The highest seed oil content was 41.87% while seeds of 14 genotypes recorded above average (32.11%) for the trait. Seed oil and protein content exhibited a significant positive correlation and moderate heritability. Out of the initially selected twenty-five random primers, twenty-two RAPD primers were found to be highly reproducible and produced a total of 183 loci of which 147 (80.32%) loci were polymorphic. Percentage of polymorphism varied from 44% to 100% with an average of 80.62%. High level of genetic variation was found among different genotypes of P. pinnata. Both molecular and oil content (biochemical) markers appeared useful in analyzing the extent of genetic diversity in Pongamia and the result of these analyses will help to better understand the genetic diversity and relationship among populations. Overall, the Pongamia genotypes included in the study showed a correlation with their geographical origins such that genotypes from the same region tend to have higher genetic similarity as compared to those from different regions. However, in UPGMA based Nei’s analysis, some genotypes were found not to be grouped based on geographical origins possibly due to the exchange of germplasm over time between farmers across the regions. The results from oil content analyses showed that several genotypes in ‘Central and Western Plateau’ agroclimatic zone of Jharkhand displayed a good potential for high oil content. The study provides insight about P. pinnata populations in Jharkhand (India) and constitutes a set of useful background information that can be used as a basis for future breeding strategy and improvement of the species.

목차

Abstract
 Introduction
 Materials and Methods
  Plant material
  Biochemical analysis
  DNA extraction
  RAPD analysis
  Data analysis
 Results and Discussion
  Biochemical characteristics
  Molecular characterization
 Conclusion
 References

키워드

저자

• Kanchan Kumari [ Molecular Biology and Tree Physiology Lab, Botany, Silviculture and NWFP Division, Institute of Forest Productivity, Ranchi-835303, India ]
• Amrita Sinha [ Molecular Biology and Tree Physiology Lab, Botany, Silviculture and NWFP Division, Institute of Forest Productivity, Ranchi-835303, India ]
• Sanjay Singh [ Molecular Biology and Tree Physiology Lab, Botany, Silviculture and NWFP Division, Institute of Forest Productivity, Ranchi-835303, India ] Corresponding author
• B.N. Divakara [ Institute of Wood Science and Technology, Malleswaram, Bangalore-560 003, India ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Journal of Forest and Environmental Science [산림과학연구]

• 간기

  계간

• pISSN

  2288-9744

• eISSN

  2288-9752

• 수록기간

  1981~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 526 DDC 634