Earticle

초록

영어

There are two tree frog species of genus Hyla in Korea: H. japonica and H. suweonensis. Hyla suweonensis, is an endemic species and is designated as an endangered species I in 2012 by the Korean Ministry of Environment. Hyla suweonensis populations are decreasing rapidly due to habitat destruction and human activities. To maintain the biodiversity in South Korea, a strategic conservation plan is urgently needed. The purposes of this study were to investigate the genetic diversity of H. suweonensis and to provide basal genetic information, using mitochondrial cytochrome b (cyt b) gene and cytochrome oxidase subunit I (COI) gene analyses. Also, we examined intraspecific genetic diversity of H. japonica and genetic divergence between H. suweonensis and H. japonica. We used samples from South and North Korea, Russia, and Japan. Partial sequences of cyt b (926 bp) and COI (534 bp) genes were obtained from 68 H. suweonensis and 271 H. japonica individuals. Hyla suweonensis was also identified in North Korea using molecular data. Most populations of two species had unique haplotypes with few nucleotide substitutions in both genes. Mean nucleotide diversity (π) of H. suweonensis was relatively lower than that of H. japonica for both genes. The average sequence divergence of combined cyt b and COI genes among Korean H. suweonensis and H. japonica populations were 0.002 and 0.009, respectively. The mean sequence divergence between H. suweonensis and H. japonica was 0.137, indicating genetic differentiation occured approximately 7 million years ago, based on 2% divergence rate per million year.

목차

Abstract
 Introduction
 Materials and Methods
  1. Sample Collection
  2. DNA extraction, PCR amplification, and Mitochondrial DNA sequencing
  3. Data Analysis
 Results and Discussion
  1. Mitochondrial markers
  2. Genetic diversity and genetic differentiation
  3. Implications for conservation of Hyla suweonensis
 Acknowledgment
 References

키워드

저자

• Suwon Chun [ Conservation Genome Resource Bank for Korean Wildlife and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University ]
• Eugene Chung [ Conservation Genome Resource Bank for Korean Wildlife and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University ]
• Inna Voloshina [ Lazovsky State Nature Reserve, 56 Centralnaya St., Lazo, Primorsky Krai 692980, Russia ]
• Jong Ryol Chong [ Wildlife Research Center, Korea University, Kodaira 1-700, Japan ]
• Hang Lee [ Conservation Genome Resource Bank for Korean Wildlife and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University ]
• Mi-Sook Min [ Conservation Genome Resource Bank for Korean Wildlife and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국양서ㆍ파충류학회지 [Korean Journal of Herpetology]

• 간기

  연간

• pISSN

  2005-9019

• 수록기간

  2009~2025

• 십진분류

  KDC 497 DDC 590