Earticle

초록

영어

This research was to investigate the inhibitory effects of tyrosinase, elastase and collagenase using rapeseed meal extract for the functional cosmetics. Gamma-tocopherol and alpha-tocopherol contents were 304.9 and 212.2 mg/kg, respectively. In the case of delta-tocopherol and plastochromanol- 8, they were 12.1 and 35.7 mg/kg, respectively. The total phenol content of methanol extract was the highest (49.6 mg/g) which was about 4.96 fold higher than that of water extract. The maximum nitrite scavenging activities of methanol and acetone extract at pH 1.2 were 85.2 and 80.1%, respectively, at 8.0 mg/mL. When the extract concentration of rapeseed meal increased upto 2.0 mg/mL, cell viabilities did not appear to have any significant cytotoxic effect, irrespective of extracts. Tyrosinase and elastase inhibition activities increased from 25.2 to 42.5% and 25.3 to 48.0%, respectively, as methanol extract concentration increased from 0.5 to 1.0 mg/mL. The collagenase inhibition activities of methanol and acetone extracts at 1.0 mg/mL were 67.2 and 68.0%, respectively. These results showed that the methanol and acetone extract of rapeseed meal can be used as a new source of functional cosmetic agent.

목차

Abstract
 1. 서론
 2. 재료 및 방법
  2.1.시료
  2.2.열수 추출물
  2.3. 메탄올 추출물
  2.4. 아세톤 추출물
  2.5. 토코페롤 분석
  2.6. 폴리페놀
  2.7. 추출 수율
  2.8. 아질산염 제거 효과
  2.9. 세포 생존율 측정
  2.10. 멜라닌세포 배양방법
  2.11. 멜라닌 함량
  2.12. Tyrosinase 활성 저해
  2.13. Elastase 활성 저해
  2.14. Collagenase 활성 저해
 3. 실험 결과
  3.1. 토코페롤
  3.2. 폴리페놀
  3.3. 추출 수율
  3.4. 세포 생존율
  3.5. 추출물 pH 변화에 따른 아질산염 제거 효과
  3.6. 추출물 농도에 따른 아질산 제거 효과
  3.7. 멜라닌 합성 억제
  3.8. Tyrosinase 활성 저해능
  3.9. Elastase 저해 활성
  3.10. Collagenase 저해 활성
 4. 결론
 REFERENCES

키워드

저자

• 김선미 [ Sun Mi Kim | 조선대학교 산업대학원 미용향장학과 ]
• 나명순 [ Myung Soon Na | 조선대학교 산업대학원 미용향장학과 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576