Earticle

초록

영어

The objective of this study was to examine the effects of high concentrations of glucose on porcine parthenotes developing in vitro. Addition of 55 mM glucose to the culture medium of embryos at the four-cell-stage significantly inhibited blastocyst formation, resulting in fewer cells in blastocyst-stage embryos and increased levels of apoptosis and autophagy compared to control. Quantitative reverse transcriptase (RT) PCR analysis revealed that the expression of pro-apoptotic genes (Caspase 3, Bax and Bak) and autophagy genes (Atg6 and Atg8/Lc3) were increased significantly by the addition of 55 mM glucose to the culture medium compared to control. MitoTracker Green fluorescence revealed a decrease in the overall mitochondrial mass compared to control. However, the addition of 55 mM glucose had no effect on mRNA expression of the nuclear DNA-encoded mitochondrial-related genes, cytochrome oxidase (Cox) 5a, Cox5b and Cox6b1. These results suggest that hyperglycemia reduced the mitochondrial content of porcine embryos developing in vitro and that this may hinder embryonic development to the blastocyst stage and embryo quality by increasing apoptosis and autophagy in these embryos.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Oocyte Collection and In Vitro Maturation
  Embryo Culture
  Terminal Deoxynucleotidyl Transferase-Mediated dUTPNick-End Labeling (TUNEL) AssaTerminal Deoxynucleotidyl Transferase-Mediated dUTP Nick-End Labeling (TUNEL) Assayy
  Mitochondrial Staining and Image Analysis
  Immunofluorescence Staining
  Real Time Reverse Transcription Polymerase Chain Reaction(Real Time RT-PCR)
  Statistical Analysis
 RESULTS
  Effect of Different Concentrations of Glucose on PorcineEmbryos Developing In Vitro
  Glucose Concentration Affects Cell Number and Apoptosisin The Blastocyst Stage
  Hyperglycemia Affects Mitochondrial Distribution but not Nuclear DNA-Encoded Mitochondrial Gene Expressionin Blastocysts
  Effect of Hyperglycemia on Autophagy and Autophagy-Related Gene Expression in Blastocysts
 DISCUSSION
 REFERENCES

키워드

저자

• Yong-Nan Xu [ Department of Animal Sciences, Chungbuk National University ]
• Ying-Hua Li [ Department of Animal Sciences, Chungbuk National University ]
• Sung Hyun Lee [ Department of Animal Sciences, Chungbuk National University ]
• Jung-Woo Kwon [ Department of Animal Sciences, Chungbuk National University ]
• Seul Ki Lee [ Department of Animal Sciences, Chungbuk National University ]
• Young-Tae Heo [ Department of Animal Sciences, Chungbuk National University ]
• Xiang-Shun Cui [ Department of Animal Sciences, Chungbuk National University ]
• Nam-Hyung Kim [ Department of Animal Sciences, Chungbuk National University ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636