Earticle

초록

영어

The aim of this study was to examine inhibitory effects of pine needle ethanol extracts (PNEE) on atopic dermatitis (AD). To determine inflammatory activity PNEE was added to LPS-induced murine peritoneal macrophages for an in-vitro test. In addition, anti-AD test was carried out by spreading PNEE on the dorsal skin of 2,4-dinitrochlorobenzene (DNCB)- induced BALB/c mice. It was confirmed that the nitric oxide (NO) secretion was suppressed when 1~ 50 μg/mL of PNEE were added to LPS-induced murine peritoneal macrophages. Moreover, levels of TNF-α, IL-6, and IL-1β‚ were decreased. For the anti-AD test, PNEE alleviated symptoms of the erythema in DNCB-induced mice. Furthermore, the IFN-γ secretion of the group treated with PNEE was increased in splenocytes from DNCB-induced mice compared to the positive control, while IL-4 secretion diminished. Through these results, we can conclude that PNEE can inhibit AD by modulating the IFN-γ, IL-4 cytokines production and inhibiting inflammation.

목차

Abstract
 1. 서론
 2. 재료 및 방법
  2.1. 재료
  2.2. 실험동물
  2.3. pH 및 색도
  2.4. 대식 세포 분리 및 배양
  2.5. 대식 세포의 nitric oxide 분비량 측정
  2.6. 대식 세포의 cytokine 분비량 측정
  2.7. 아토피 피부염 유발 및 시료 처리
  2.8. 비장 세포 분리 및 배양
  2.9. 비장 세포 배양액의 cytokine 분비량 측정
  2.10. 혈청의 total IgE 함량 측정
  2.11. 비장 세포의 증식능 측정
  2.12. 통계 처리
 3. 결과 및 고찰
  3.1. pH 및 색도
  3.2. 대식 세포의 nitric oxide 분비량
  3.3. 대식 세포의 cytokine 분비량
  3.4. 비장 세포 배양액의 cytokine 분비량
  3.5. 혈정의 total IgE 함량
  3.6. 비장 세포의 증식능 측정
  3.7. 아토피 피부염 증상
 4. 결론
 감사
 REFERENCES

키워드

저자

• 정다현 [ Da-Hyun Jeong | 부경대학교 식품공학과/식품연구소 ]
• 김꽃봉우리 [ Koth-Bong-Woo-Ri Kim | 부경대학교 수산과학연구소 ]
• 정슬아 [ Seul-A Jung | 부경대학교 식품공학과/식품연구소 ]
• 김현지 [ Hyun-Jee Kim | 부경대학교 식품공학과/식품연구소 ]
• 강보경 [ Bo-Kyeong Kang | 부경대학교 식품공학과/식품연구소 ]
• 박시우 [ Si-Woo Bark | 부경대학교 식품공학과/식품연구소 ]
• 김태완 [ Tae-Wan Kim | 안동대학교 식품생명공학과 ]
• 안동현 [ Dong-Hyun Ahn | 부경대학교 식품공학과/식품연구소 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576