This study aimed to investigate the enzymatic hydrolysis of mannitol using Viscozyme® L, Celluclast® 1.5 L, Saczyme®, Novozym®, Fungamyl® 800 L, Driselase® Basidiomycetes sp., and Alginate Lyase, and to optimize of reaction conditions for production of reducing sugar. Response surface methodology (RSM) based on central composite rotatable design was used to study effects of the independent variables such as enzyme (1-9% v/w), reaction time (10-30 h), pH (3.0-7.0) and reaction temperature (30-70oC) on production of reducing sugar from mannitol. The coefficient of determination (R2) of Y1 (yield of reducing sugar by Viscozyme ® L) and Y3 (yield of reducing sugar by Saczyme®) for the dependent variable regression equation was analyzed as 0.985 and 0.814. And the p-value of Y1 and Y3 showing 0.000 and 0.001 within 1% (p < 0.01), respectively, was very significant. The optimum conditions for production of reducing sugar with Viscozyme® L were 9.0 % (v/w) amount of enzyme, 30.0 hours of reaction time, pH 4.5 and 30.0oC of reaction temperature, and those with Saczyme® were 9.0% (v/w) of amount of enzyme dosage, 30.0 h of reaction time, pH 7.0 and 30.0oC of reaction temperature, consequently, the predicted reducing sugar yields were 22.5 and 27.9 mg/g-mannitol, respectively.
목차
Abstract 1. 서론 2. 재료 및 방법 2.1. 실험재료 2.2. 실험방법 2.3. 실험계획 2.4. 통계분석 2.5. 분석방법 3. 결과 및 고찰 3.1. 효소 가수분해 3.2. Viscozyme® L를 이용한 만니톨의 가수분해 3.3. Saczyme®를 이용한 만니톨의 가수분해 4. 결론 감사 REFERENCES
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동