Earticle

초록

영어

The present study was conducted to examine the effect of antioxidant treatment during parthenogenetic activation procedure on the reactive oxygen species (ROS) levels and in vitro development of porcine parthenogenetic embryos. Porcine in vitro matured oocytes were activated by a combination of electric stimulus and 2 mM 6- dimethylaminopurine (6-DAMP) before in vitro culture. During the activation period, oocytes were treated with 50 μM β-mercaptoethanol (β-ME), 100 μM L-ascorbic acid (Vit. C) or 100 μM L-glutathione (GSH). To examine the ROS level, porcine parthenogenetic embryos were stained in 10 μM dichlorohydrofluorescein diacetate (H2DCFDA) dye 20 h after culture, examined under a fluorescence microscope, and the fluorescence intensity (pixels) were analyzed in each embryo. The parthenogenetic embryos were cultured for 6 days to evaluate the in vitro development. The apoptosis was measured by TUNEL assay. The H2O2 levels of parthenogenetic embryos were significantly lower in antioxidant treatment groups (26.9±1.6～29.1±1.3 pixels/embryo, p<0.05) compared to control (33.2±1.7 pixels/embryo). The development rate to the blastocyst stage was increased in antioxidant treatment groups (32.0～32.5%) compared to control (26.9%, p<0.05), although, there was no difference in apoptosis among groups. The result suggests that antioxidant treatment during parthenogenetic activation procedure can inhibit the ROS generation and enhance the in vitro development of porcine parthenogenetic embryos.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  In Vitro Maturation of Oocytes
  Parthenogenesis and In Vitro Culture
  Antioxidant Treatment
  Measurement of Intercellular ROS Content
  TUNEL Assay
  Statistical Analysis
 RESULTS
  H2O2 Levels of Parthenogenetic Embryos
  In Vitro Development of Parthenogenetic Embryos
  Apoptosis in Blastocysts
 DISCUSSION
 REFERENCES

키워드

저자

• Hyo-Kyung Bae [ College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University ]
• Soo-Hyun Kim [ College of Animal Life Science, Kangwon National University ]
• Sung-Young Lee [ College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University ]
• In-Sun Hwang [ College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University ]
• Choon-Keun Park [ College of Animal Life Science, Kangwon National University ]
• Boo-Keun Yang [ College of Animal Life Science, Kangwon National University ]
• Hee-Tae Cheong [ College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636