Earticle

초록

영어

Artificial insemination (AI) has been performed widely in swine industry using fresh liquid sperm instead of frozen type of sperm. However fresh sperm are not able to preserve more than three days with optimal motility and other sperm parameters for the successful fertilization, since in vitro stored sperm has an oxidative stress that resulted increase of abnormality and acrosome reation. To overcome these major problems, novel preservative formulation is needed to neutralize the oxidative stress and to provide suitable physiological environment for sperm in in vitro. In this study, naturally derived substances such as Poncirus trifoliate (Trifoliate orange), Garcinia mangostana (Mangosteen), pig placenta and testis extracts were tested as sperm preservative agents. Placenta extracts (PE), trifoliate orange extracts (TOE), testes extracts (TE) and mangosteen extracts (ME) were applied to analyze specific parameters for sperm motion characteristics individually and combinatorial. Each individual extract treatment can accelerate the sperm motility but noticeably TOE, TE and ME treatments exhibited the considerable and significant preservation of sperm motility. PE, TE and ME showed a significant (p<0.05) increase in ALH after one week. Further we evaluated the five different combinations of these extracts on sperm motility and its motion characteristics. Surprisingly even after one week ME, TOE and TE combination significantly preserved the sperm motility about 75%. It is noteworthy that unlike individual extract treatment, combination of ME, TOE and TE simultaneously protect the sperm motility and its motion characteristics. Taken together these data conclude that addition of ME, TOE and TE can be effective for preservation of pig sperm.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Preparation of Semen and Extracts
  Evaluation of Sperm Parameters
  Statistical Analysis
 RESULTS
 DISCUSSION
 REFERENCES

키워드

저자

• Woo Tae Ha [ Department of Animal & Food Bioscience, College of Natural Science, Konkuk University ]
• Won Young Lee [ Department of Animal & Food Bioscience, College of Natural Science, Konkuk University ]
• Ran Lee [ Department of Animal & Food Bioscience, College of Natural Science, Konkuk University ]
• Jae Hwan Kim [ Departmemt of Biomedical Science, College of Life Science, CHA University ]
• Nam Hyung Kim [ Department of Animal Science, College of Agriculture, Chungbuk National University ]
• Jin Hoi Kim [ Department of Animal Biotechnology, Konkuk University ]
• Il Joo Lee [ R&D Team, Darby Genetics Inc. ]
• Hyuk Song [ Department of Animal & Food Bioscience, College of Natural Science, Konkuk University ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636