Two-component signal transduction systems, which consist of a histidine sensor kinase and a response regulator, are gaining increasing interests as a useful bioparts to construct artificial genetic regulatory circuits for synthetic biology. In the present study, we investigated the possibility to use a heterologous two-component signal transduction system to fabricate an E. coli biosensor. First, we constructed an inducible expression vector containing todS sensor kinase and todT response regulator genes of the aromatic compounds-sensing TodST system of Pseudomonas putida. To use as a reporter strain,a transcriptional fusion between the TodT regulatable promoter of P. putidatodX gene and beta-galactosidase gene was then integrated into the chromosome of an E. coli strain. The beta-galactosidase activity of the reporter strain harboring TodST expression vector was strongly induced by TodST in the presence of various aromatic compounds, demonstrating the possibility to employ TodST system as a backbone for artificial aromatic compounds signal transduction system. Supported by the Intelligent Synthetic Biology Global Frontier Program, and the Next-Generation BioGreen 21 Program.
Eun-Gyeong LEE [ Systems and Synthetic Biology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Korea. ]
Abhijeet SHILLAK [ Systems and Synthetic Biology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Korea. Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, 305-350, Korea. ]
Doo-Byoung OH [ Systems and Synthetic Biology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Korea. Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, 305-350, Korea. ]
Ohsuk KWON [ Systems and Synthetic Biology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Korea. Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, 305-350, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동