Earticle

초록

영어

Recently, microbial chitinase were widely used in various processes including agricultural, biological and environmental fields, especially, to control plant pathogenic fungal. Chitinases cleaves the b (1-4) glyscosidic linkage of N-acetly-D-glucosamine present in the fungal cell wall. In the present study, 10 different chitinase producing bacterial sps. were isolated from soil samples collected at barren losses agricultural soil (Cabbage, potato and beans field). To obtain enhanced chitinase producing bacterial sps., was performed using 0.5% swollen chitin as carbon source with modified M9 media. The temperature, pH and incubation time was followed as: 30°C, 6.8 and seven days respectively. These enhanced samples were used to screen the chitinase producing bacterial with clear zone appearance in the chitin agar plates. The genomic DNA extracted from the isolated colonies was used to identify the chitinase producing gene. Further, biochemical tests, 16srDNA amplification and sequencing were carried out to identify the organism. From the results, there are 6 Pseudomonas sps., 3 Stenotrophomonas sps., and one Paenibacillus sp. were obtained with higher chitinase producing bacteria sps.

키워드

저자

• Ki Moon BONG [ Dept. of Environmetal Engineering, Chosuin University, Gwangju, 501-759. ]
• Jong Min KIM [ Dept. of Environmetal Engineering, Chosuin University, Gwangju, 501-759. ]
• Mathur Nadarajan KATHIRAVAN [ Dept. of Environmetal Engineering, Chosuin University, Gwangju, 501-759. ]
• Si Wouk KIM [ Dept. of Environmetal Engineering, Chosuin University, Gwangju, 501-759. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576