Earticle

초록

영어

Long-chain ω-hydroxy fatty acids are widely being used in many industrial fields due to its two functional groups, unlike other natural fatty acids. Chemical synthesis of ω-hydroxy fatty acids from monocarboxylic acid, palmitic acid for example, however, is not appropriate since the primary carbon has less reactivity than the secondary carbon. Currently, the production of ω-hydroxy fatty acids relies on biotransformation by Candida tropicalis. The problems with this method are that eukaryotes are not able to grow and produce desired compounds fast, and C.tropicalis ultimately oxidizes ω-hydroxy fatty acids to α,ω-dicarboxylic acids. Therefore, we expressed CYP153A from Marinobacter aquaeolei, CamA, and CamB in E. coli BL21(DE3) to overcome existing limitation. Through resting cell reaction in shaking flask, 1 mM of hexadecanoic acid was converted to 16-hydroxyhexadecanoic acid with 90.4 % yield in 6 hours. Biphasic reaction was then performed to extract the product simultaneously and increase initial substrate concentration. Various solvents were screened in terms of log POW, partition coefficient of the product, and productivity.

키워드

저자

• Jin-hyung BAE [ School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151-742. ]
• Eun-ok JUNG [ School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151-742. ]
• Da-hye JUNG [ School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151-742. ]
• Won-ji CHOI [ School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151-742. ]
• Chang-min SUNG [ School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151-742. ]
• Byung-Gee KIM [ School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151-742. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576