Earticle

초록

영어

Many researches have been focused on the development of cellulases having high catalytic efficiency on crystalline cellulose and thermo-stability at elevated temperature to obtain fermentable sugars for bio-ethanol source from the most abundant plant biomass. Previously, we produced a cellulase of Clostridium thermocellum (C-cellulase) whose structure has been elucidated in recombinant Escherichia coli BL21 displaying a high soluble expression and a superior activity at 50~55 oC. However, the stability of C-cellulase decreased rapidly above 55 oC. In this study, we investigate the two strategies for improving C-cellulase; the first is to obtain a new cellulase which can be evolved to more efficient cellulase by protein engineering and the second is to increase the thermo-stability of C-cellulase at a higher temperature by fusing it to maltose-binding protein (MBP), glutathionestransferase (GST) and ferritin (Fn), respectively. We searched a new cellulase-like protein from Clostridium sp.L2-50 which shows identity with C-cellulase, resulting in a high soluble expression in E. coli. The PCR fragment of C-cellulase was cloned into the C-terminus of MBP gene using pMAL-2x, producing MBP-C-cellulase or the C-terminus of GST gene using pET 42b+ vector, producing GST-C-cellulase. The gene of C-cellulase was fused to N terminus of ferritin gene which was already cloned in pETDuet vector, producing C-cellulase-Fn.

키워드

저자

• Seung Pil PACK [ Dept. of Biotechnology and Bioinformatics, Korea University, Sejong 339-700, Korea. ]
• Young Ha RYU [ Dept. of Biotechnology and Bioinformatics, Korea University, Sejong 339-700, Korea. ]
• Ki Baek YEO [ Dept. of Biotechnology and Bioinformatics, Korea University, Sejong 339-700, Korea. ]
• Ki Ha MIN [ Dept. of Biotechnology and Bioinformatics, Korea University, Sejong 339-700, Korea. ]
• Eui Kyung JANG [ Dept. of Biotechnology and Bioinformatics, Korea University, Sejong 339-700, Korea. ]
• Joo Hee JANG [ Dept. of Biotechnology and Bioinformatics, Korea University, Sejong 339-700, Korea. ]
• Mi-Ran KI [ Dept. of Biotechnology and Bioinformatics, Korea University, Sejong 339-700, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576