Earticle

초록

영어

For production of biomass and protease, statistical experimental methods were applied to optimize production–medium composition using a Bacillus licheniformis SCK 121057. Firstly, medium components thought to have positive effects on biosynthesis of biomass and protease were selected by preliminary experiments; one factor at a time method. Factors showing significant effects were chosen by the Plackett-Burman design. Fractional factorial design (FFD) was then used to investigate the interactive effects of the selected medium components on productivity of biomass and protease. The steepest ascent method (SAM) and response surface methodology (RSM) were employed to determine the optimal concentrations of each component for maximal production of biomass and protease. Using the optimized medium, 4.02 g/L of biomass and 161.27 U/ml of protease were produced, almost three times higher than that from the initial medium conditions. Finally, fermentation conditions were examined for production of biomass and protease in a laboratory fermentor using the optimized production medium. The maximum biomass (6.54 g/L) and protease (240.06 U/ml) were obtained at 42℃, 200 rpm, 2 vvm and pH 8.

키워드

저자

• So Yeon IM [ Department of Food Science & Technology, Chonbuk National University, South Korea. ]
• Do Yeon JEONG [ Sunchang Research Center for Fermentation Microbes, South Korea. ]
• GieTaek CHUN [ Department of Life Science, Kangwon National University, South Korea. ]
• Yong Seob JEONG [ Department of Food Science & Technology, Chonbuk National University, South Korea. ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576