Earticle

초록

영어

Fractional precipitation is a simple, efficient method for purifying paclitaxel extracted from plant cell cultures. However, fractional precipitation process has been inherently problematic due to the lengthy precipitation time that is required. An improved fractional precipitation process could significantly reduce the precipitation time by increasing the surface area available for precipitation. Silica-alumina was used to increase the surface area, and the optimal surface area per working volume (i.e. volume of reaction solution) (S/V) for achieving the highest purity and yield of paclitaxel possible was found to be 1007.6 mm-1. In this study, we evaluated the effects of the zeta potential of silica-alumina on the behavior (purity, yield, fractional precipitation time, precipitate shape and size) of fractional precipitation for improving the purification efficiency of paclitaxel. As the zeta potential of surface area-increasing materials were increased, the yield of paclitaxel was increased and the precipitation time and precipitate size were decreased. Furthermore, we also found that the purity of paclitaxel was constant regardless of the zeta potential of silica-alumina.

키워드

저자

• Heung-Kon RYU [ Dept. of Chemical Engineering, Kongju NationalUniversity, Cheonan 330-717, Korea. ]
• Jin-Hyun KIM [ Dept. of Chemical Engineering, Kongju NationalUniversity, Cheonan 330-717, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576