Yoo Min PARK, Su Jin KIM, Jae Ho YOON, Hyun C. YOON
언어
영어(ENG)
URL
https://www.earticle.net/Article/A197691
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원문정보
초록
영어
An optical biosensor for the osteoarthritis (OA) diagnosis based on the quantification of OA biomarker in human serum and urine was developed. In the OA progresses, the substances of joint such as cartilage protein are degraded and secreted into serum and urine. These cartilage degradation proteins such as C-telopeptide of type II collagen (CTX-II) are used as significant OA biomarker. The CTX-II is classified as serum CTX-II (sCTX-II) and urine CTX-II (uCTX-II) according to its existence site. Due to the structural differences between sCTX-II (homo-dimer) and uCTX-II (monomer), two different types of immunoassays such as competitive immunoassay for uCTX-II and sandwich assay for sCTX-II are employed. For the simultaneous quantification analysis of these markers, we developed a fluoromicrobeadbased multiplex guiding chip [1]. On the chip interface, CTX-IIs are bio-specifically captured and its aspect is appeared as formation of fluorescent dot. By counting the number of bead on sensing surfaces, the concentrations of CTX-II were quantified. The obtained optical signal showed good correlation with sCTX-II concentration from 6.3 to 250 pg/ml and uCTX-II concentration from 1.4 to 10 ng/ml.
키워드
biosensorbiochiposteoarthritis
저자
Yoo Min PARK [ Department of Applied Chemistry & Biotechnology, Ajou University. ]
Su Jin KIM [ Department of Applied Chemistry & Biotechnology, Ajou University. ]
Jae Ho YOON [ Department of Applied Chemistry & Biotechnology, Ajou University. ]
Hyun C. YOON [ Department of Applied Chemistry & Biotechnology, Ajou University. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동