Earticle

초록

영어

A yhcN promoter based bioreporter strain was developed for the detection of phenolics in plant hydrolysates. Previous microarray data suggest that the yhcN gene was highly expressed when exposed to phenolics. To confirm this, RT-qPCR was performed and result shows that this gene was significantly up-regulated when E.coli BL21 (DE3) exposed for 10 min to 500 ppm of ferulic acid (7.2 fold) or vanillin (9.7 fold) furfural (2.3 fold), Consequently, an E. coli yhcN::lux bioreporter strain was and the resultant strain was analyzed during an exposure to hydrolysate-related phenolics and a rice hydrolysate sample. The strain had a maximum response at the time of 180 min. The strain is very responsive to furfural (25-fold) and vanillin (21-fold). The responses with different acids were also significant, with relative responses of between 4- and 16-fold. The minimum detectable concentrations were 156.12 mg/L for both furfural and 5-hydroxymethyl furfural. In tests with rice hydrolysate samples, a maximum response of 3.5 was obtained and the minimum detectable phenolics concentration, as based upon Folin-Cioalteau’s reagent, was 39 mg/L phenolics. These results illustrate that this strain can be used to monitor for the presence of chemicals within plant hydrolysate samples that may be potentially inhibitive toward downstream fermentative processes.

키워드

저자

• Ajay Kalanjana MONNAPPA [ School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology, 50-UNISTgil, Banyeon-ri, Eonyang-eup, Ulsan 689-805, Republic of Korea. ]
• Jin Hyung LEE [ Institute of Engineering Thermophysics, Chongqing University, Chongqing, 400030, China. ]
• Robert James MITCHELL [ School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology, 50-UNISTgil, Banyeon-ri, Eonyang-eup, Ulsan 689-805, Republic of Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576