An efficiency pressurized liquid extraction (PLE) coupled with high performance liquid chromatography (HPLC) for quantification of biological compound tricin in black bamboo leaves (Phyllostachys nigra) was described in this study. The environmentally and health-friendly green solvents ethanol and water were used for PLE extraction. Parameters affecting extraction were optimized using the univariate method. The best extraction conditions 200℃, 50% ethanol, 20 min static time and 425 μm particle size. Compared with the traditional reflux extraction method, the optimized PLE condition showed high extraction efficiency 249 mg/100g dry leaves and it’s 19 times higher than reflux method, while taking less time and employing fewer solvents. The calibration curve for tricin was established with good linearity and correlation coefficient (r) 0.9997. To the best of our knowledge, this study is the first contribution to utilize PLE for tricin extraction. The experiment results indicated that the PLE is a promising prospect in extracting quantities tricin in plant, which can be used for pharmaceutical/biomedical applications and evaluation.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동