Earticle

초록

영어

In this study, we propose a micro/nanofluidic device for the rapid quantification of bacteria (Escherichia coli). This device is comprised of a micromixer and a preconcentrator. The micromixer is used for mixing bacterial cells with tagging molecules and can be easily integrated to the preconcentrator and fabricated by polydimethylsiloxane (PDMS) replica molding method. The preconcentrator consists of two microchannels (main and sub) that are connected by the nanochannels, which can be easily fabricated by electric breakdown of a ~25 μm-thick polydimethylsiloxane (PDMS) membrane using high electric shock. For quantification of bacteria, bacteria sample is firstly mixed with fluorescent dye and continuously preconcentrated at the target position by applying the electric field through the junction of micro- and nanochannels. The concentration of bacterial sample can be quantified by measuring the fluorescence intensity at the preconcentrated region. We believe that this micro/nanofluidic biosensor will be useful for bacteria detection.

키워드

저자

• Zhongwei WANG [ Department of Mechanical Engineering, Inha University, Incheon ]
• Tae-Joon JEON [ Department of Biological Engineering, Inha University, Incheon ]
• Sun Min KIM [ Department of Mechanical Engineering, Inha University, Incheon ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576