Among feedstock of biodiesel, oleaginous yeasts have been known for outstanding abilities to grow fast and accumulate large lipid in their cells. In this study, oleaginous yeasts were isolated from the carbon abundant environments, mine area, an found optimal characteristics. Finally, 6 species were identified by performing 18S rDNA sequencing, two Saccharomyces sp., Saccharomyces cerevisiae, fungal sp., Williopsis saturnus,andTorulaspora delbrueckii. Each lipid-body of sample was visualized by confocal fluorescence microscope with nile-red solution. In order to find optimal characteristic, three culture conditions were regulated such as carbon sources, pH, and temperature (3). As a result, all the isolated yeast grew and accumulated lipid differently. The specific growth rates were observed from 0.31h-1 to 0.38h-1. On each fast grow conditions, lipid contents were measured from 5% to 11%(not optimized) (1, 2). This study confirmed that the isolated yeasts from mine area can grow and accumulate lipid. Therefore, by regulating culture condition and finding other better substrates, isolated yeasts will be promising feedstock of biodiesel.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동