Earticle

초록

영어

Microalgae have emerged as 3rd generation biomass for biodiesel production. However, despite importance of genetic approaches for strain improvement, molecular tools have limited. Based on this background, our research have focused on developing advanced vector systems containing inducible promoter. The main advantage of utilizing inducible promoter elements is that the gene expression of an organism can be selectively manipulated via alteration of its surroundings. Our study aimed at the construction of vector construct with inducible promoter elements which would in turn be transformed to Chlamydomonas reinhardtii to investigate the effects of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) overexpression. The inducible Chlamydomonas promoter nitrate reductase (Nia1), which is activated by NO3- and repressed by NH4+ was used to drive the overexpression of gapA subunit of the G3PDH protein. pCr102 vector was used as a main back-bone vector, and the existing PSAD promoter was switched with nia1. Then, Glyceraldehyde 3-phosphate dehydrogenase (gapA) element was inserted to produce a new recombinant vector, pCrN1G3. The construct will be transformed into C. reinhardtii protoplast via glass bead method. Here, we expect that the lipid content will be increased under the presence of nitrate by the overexpression of gapA gene.

키워드

저자

• Sung-Eun SHIN [ Dept. of Chemical and Biomolecular Engineering, KAIST, Daejeon, 305-701. ]
• Gang-Guk CHOI [ Dept. of Chemical and Biomolecular Engineering, KAIST, Daejeon, 305-701. ]
• Eun Kyung KIM [ Advanced Biomass R&D Center, #2502 Building W1-3, KAIST, Daejeon, 305-701. ]
• Bongsoo LEE [ Dept. of Chemical and Biomolecular Engineering, KAIST, Daejeon, 305-701. ]
• Min S. PARK [ Advanced Biomass R&D Center, #2502 Building W1-3, KAIST, Daejeon, 305-701. ]
• Ji-Won YANG [ Advanced Biomass R&D Center, #2502 Building W1-3, KAIST, Daejeon, 305-701. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576