Energy insecurity and global warming are the biggest challenges for the global economy. Overall, transportation sector is the biggest consumer of nonrenewable fossil fuels. Bioethanol is a practical green alternative transportation fuel which has numerous advantages over conventional fossil fuels. However, grains such as corns are used as the feedstock for commercial bioethanol which may cause food insecurity. Therefore, it is vital to produce cellulosic ethanol from non-food biomass sources. However, these materials are quite recalcitrant to be converted into fermentable sugars due to its complex structure. To overcome this barrier, in this research, we searched for new microorganism species by isolating cellulose degraders with high efficiency. Instead of random sampling, several sources were targeted in the nature where cellulosic material was decomposed such as Suncheon bay, silkwarm, termite, and excrement of various grass-eating animals. Total 48 species which showed cellulose degrading ability were isolated both in aerobic and anaerobic conditions by ten-fold serial dilution, heat treatment, and cellulase activity assay with filter paper and Congo red. Several samples from grass-eating animals showed high cellulose-degrading efficiency. In enrichment, five pieces of filter paper were completely degraded and clearing zone around colonies was formed in Congo red plates. The strains of N-b and N-d from Nyala were determined as Pleomorphomonas sp. and Cellulomonas sp. by 16s rDNA sequencing. These strains showed highest growth rate in laboratory-based minimal nutrient broth with glucose, xylose, and CMC as carbon source. It was also observed that both species converted more than 30 percent of the glucose into volatile fatty acid (VFA).
키워드
Cellulosic BioethanolVFA productionIsolation of Microbes
저자
Bomi LEE [ Dept. of Civil and Environmental Engineering, KAIST, Daejeon, 305-701. ]
Hoon CHO [ Dept. of Civil and Environmental Engineering, KAIST, Daejeon, 305-701. ]
Gayeong KIM [ Dept. of Civil and Environmental Engineering, KAIST, Daejeon, 305-701. ]
Jong-In HAN [ Dept. of Civil and Environmental Engineering, KAIST, Daejeon, 305-701. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동