Earticle

초록

영어

As a tool for a drug,the preparation of liposomes has been examined for several decades. We investigated to encapsulate lysosomal enzymes extracted from Saccharomyces cerevisiae in liposomes, which are made with L-α-phosphoatidylcholine from egg yolk. We mixed lipid, cholesterol and lysosomal membrane protein isolated from lysosome treated by H2O2 and NH4Cl to modify liposome surface for antimicrobial activity before and after making liposome. The residue after evaporation was mixed using lysosomal enzymes with same ratio and was vortexed to encapsulate them for 1 min. Large lamellar vesicles were sonicated to make a small unilamellar vesicles for 5 sec. The liposomes were confirmed as cell counts against Escherichia coli to evaluate the antimicrobial activity of lysosomal enzymes. The results showed that the antimicrobial activity was not shown without modification of liposome surface. Also, when the liposome was made with among lipid, cholesterol and lysosomal memebrane proteins, we got more good results. Furthermore, to increase the antimicrobial activity, when liposome was used lysosomal membrane proteins treated by H2O2, it was better than others. Therefore, lysosomal membrane proteins for targeting bacteria will play an important a role.

키워드

저자

• Seung Hyuck BANG [ Department of Bioprocess Engineering, Chonbuk National University, Jeonju, 561-756. ]
• Yang-Hoon KIM [ Deparment of Microbiology, Chungbuk National University, Cheongju, 361-763. ]
• Jiho MIN [ Department of Bioprocess Engineering, Chonbuk National University, Jeonju, 561-756. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576