Earticle

초록

영어

Levulinic acid (LA) is a versatile chemical building block that can be produced from cellulosic biomass and can be converted into valuable chemicals and fuels. In this study, enzymatic reduction of LA was performed for the synthesis of 4-hydroxyvaleric acid (4HV), which can be used as a monomer of bio-polyester and a precursor of bio-fuels. Because no enzyme that has LA as its native or major substrate has been reported, engineering of the substrate specificity of an enzyme is required to yield the enzymatic reduction of LA. 3-Hydroxybutyrate dehydrogenase (3HBDH) from Alcaligenes faecalis was selected as a target enzyme for the engineering of substrate specificity. A rational design approach with molecular docking simulation was performed for the engineering of the substrate specificity of the enzyme. Through the mutational studies of 3HBDH, the substrate specificity of the enzyme was engineered and a double mutant which has catalytic activity toward LA was selected as a best mutant. Approximately 57% conversion of LA was achieved with this mutant in 24 hr, while no conversion was achieved with the wild-type enzyme.

키워드

저자

• Young Joo YEON [ School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Republic of Korea. ]
• Young Joo YEON [ School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Republic of Korea. ]
• Hyung-Yeon PARK [ Bio-MAX Institute, Seoul National University, Seoul 151-742, Republic of Korea. ]
• Young Je YOO [ School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Republic of Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576