Earticle

초록

영어

Transgenic plant cell cultures have been used as a production system for therapeutic proteins. Rice cells were transformed to express human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig). With RAmy3D promoter system, expression of the target protein could be strongly induced by sugar starvation. However, sugar-depleted environment can cause cell death and secretion of proteases into the medium due to cell lysis. In addition, during the mass cultivation and production using bioreactors, various physical factors such as shear stress and bubble burst can damage the cells. In this study, effects of an non-ionic surfactant, Pluronic F-68 (PF-68), as a protective agent in transgenic rice cell cultures were examined to enhance the production. Various concentrations of PF-68 at 1, 3, 5, 7 g/L were added in induction phase. The best hCTLA4Ig production could be obtained with 5 g/L PF-68. It was thought that protective effects against shear stress could enhance hCTLA4Ig production in bioreactor.

키워드

저자

• Yun-Young PAK [ Dept. of Biological Engineering, Inha University, Incheon, 402-020. ]
• Jun-Young KWON [ Dept. of Biological Engineering, Inha University, Incheon, 402-020. ]
• Hyung-Jin NAM [ Dept. of Biological Engineering, Inha University, Incheon, 402-020. ]
• Min-Sub KIM [ Dept. of Biological Engineering, Inha University, Incheon, 402-020. ]
• Hong-Yeol CHOI [ Dept. of Biological Engineering, Inha University, Incheon, 402-020. ]
• Youn-Kyung SEONG [ Dept. of Biological Engineering, Inha University, Incheon, 402-020. ]
• Sun-Mi LEE [ Dept. of Biological Engineering, Inha University, Incheon, 402-020. ]
• Dong-Il KIM [ Dept. of Biological Engineering, Inha University, Incheon, 402-020. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576