Earticle

초록

영어

This study was conducted to screen the alginate-degrading microorganism and purification and characterization of alginate-degrading enzyme. The alginate-degrading marine bacteria was isolated from brown algae Sargassum thunbergii. In the result of 16S ribosomal RNA sequence analysis, the strain was identified as Vibrio sp. V140 and named as Vibrio sp. PKA 1003. The optimum culture conditions for the growth of as Vibrio sp. PKA 1003 were pH 7, 3% NaCl, 25℃ and 24 hr incubation time. To obtained the crude enzyme, as Vibrio sp. PKA 1003 was incubated its optimum culture conditions and the culture medium of the bacterium was centrifuged for 30 min at 12,000 ×g, 4℃. The supernatant was utilized as crude enzyme and the optimal conditions for alginate-degrading ability of enzyme was measured by reducing sugar assay and viscometry. The crude enzyme of alginate-degrading bacteria, as Vibrio sp. PKA 1003, showed highest level of alginate-degrading activity when cultured on pH 9, 30℃, 6% alginic acid and 63 hr incubation time. The crude alginate-degrading enzyme produced by Vibrio sp. PKA 1003 was purified by ammonium sulfate salting, dialysis, DEAE-sephadex, sephadex G-100 and Sephacryl S-200 HR column. The effects of pH, NaCl and temperature on purified enzyme and the metal and substrate specificity was measured by reducing sugar assay.

키워드

저자

• Hyun Jee KIM [ Dept. of Food Science and Technology, Pukyong National University/ Institute of Food Science, Busan, 608-737. ]
• Koth Bong Woo Ri KIM [ Institute of Fisheries Sciences/Pukyong National University, 474, Ilgwang-ro, Ilgwang-myeon, Gijang-gun, Busan. ]
• Seul A JUNG [ Dept. of Food Science and Technology, Pukyong National University/ Institute of Food Science, Busan, 608-737. ]
• Da Hyun JEONG [ Dept. of Food Science and Technology, Pukyong National University/ Institute of Food Science, Busan, 608-737. ]
• Bo Kyeong KANG [ Dept. of Food Science and Technology, Pukyong National University/ Institute of Food Science, Busan, 608-737. ]
• Si Woo BARK [ Dept. of Food Science and Technology, Pukyong National University/ Institute of Food Science, Busan, 608-737. ]
• Won Min PARK [ Dept. of Food Science and Technology, Pukyong National University/ Institute of Food Science, Busan, 608-737. ]
• Bo ram KIM [ Dept. of Food Science and Technology, Pukyong National University/ Institute of Food Science, Busan, 608-737. ]
• Sung Mee LIM [ Dept. of Food Nutrition and Science, Tongmyong University, Busan, 608-711. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576