Earticle

초록

영어

We developed depigmenting agents of inhibition of MITF (Microphthalmiaassociated transcription factor) binding and melanosome transport for promotion of cosmetics industry and technology diffusion. Our research were progressed by whitening materials selection in specific target using HTS (High throughput screening) Biochip and pharmacophore database virtual screening using molecular design methods of protein target structure. First, we built of skin whitening natural products library and database and evaluated materials by cell-based assay and artificial skin animal alternative test methods. DNA chip is to evaluate the interaction of MITF with DNA binding site E-box by SPR (Surface Plasmon resonance). So, we screened 10 MITF-DNA binding natural products through virtual screening in pharmacophore DB using Structure Based Drug Design (SBDD) and then final 3 ingredients (including N7) selection through E-box DNA and MITF binding by EMSA. In addition, we screened 26 Rab27a- Melanophilin binding inhibitors for the target database to fit the pharmacophore in KFDA natural products 270 and 350 million compounds and then, selected final 10 candidates by establishment of cell based assay for melanosome transport inhibitory activity from 280 compounds library. We developed BMD 27, and it’s containing extracts and completion prototype cosmetic products. This study will contribute to the cosmetics industry and global marketing by application of convergence of BT, IT, NT technology.

저자

• Eun Ki Kim [ Department of Bioological Engineering, Inha University ]
• Jae Sung Hwang [ Department of Genetic Engineering, Kyung Hee University ]
• Jee-Young Lee [ Bioinformatics & Molecular Design Research Center ]
• Joo Dong Lee [ Enprani Co., Ltd. R&D Center of Skin Science and Cosmetics ]
• Bora Kim [ Enprani Co., Ltd. R&D Center of Skin Science and Cosmetics ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576