Earticle

초록

영어

While subcutaneous tissue has been proposed as an optimal site for pancreatic islet transplantation, concern remains that the microvasculature of subcutaneous tissue is too poor to support transplanted islets. In an effort to overcome this limitation, we evaluated whether fibrin gel with human adipose-derived stem cells (hADSCs) and pancreatic islet cells could cure diabetes mellitus if transplanted into the subcutaneous space of diabetic mice. The fibrin gel with islet cells and hADSCs was subcutaneously implanted into diabetic mice resulting in normalization of the recipient’s blood glucose levels. These results were enhanced by co-treatment of fibroblast growth factor-2 (FGF2) in the fibrin gel. The hADSCs induced formation of new microvasculature via overexpression of vascular endothelial growth factor (VEGF), reducing apoptosis of the co-transplanted islet cells (enhancement of Bcl-2 expression in islets). The new microvasculature was likely secondary to induction by hypoxia-induced factor-1a (HIF-1a) in hADSCs, but may also be derived from recipient endothelial cells. Secondary to insulin contained within the co-transplanted islets, the hADSCs did not directly differentiate into endothelial cells (no detection of biomarkers of human endothelial cells), but instead into insulin-secreting cells (detection of human insulin). Mice receiving islet cell transplantation alone did not become normoglycemic. Collectively, cotransplantation of fibrin gel with islet cells and hADSCs will expand the indications for islet cell transplant therapy and the potential clinical application of cell-based therapy.

키워드

저자

• Suk Ho Bhang [ School of Chemical and Biological Engineering, Seoul National University, South Korea ]
• Min Jin Jung [ Departments of Bioengineering, College of Engineering, and Institute for Bioengineering and Biopharmaceutical Research, Hanyang University, Seoul 133-791, South Korea ]
• Wan-Geun La [ School of Chemical and Biological Engineering, Seoul National University, South Korea ]
• Jung-Youn Shin [ School of Chemical and Biological Engineering, Seoul National University, South Korea ]
• Yong Hwa Hwang [ Departments of Bioengineering, College of Engineering, and Institute for Bioengineering and Biopharmaceutical Research, Hanyang University, Seoul 133-791, South Korea ]
• Min Jun Kim [ Departments of Bioengineering, College of Engineering, and Institute for Bioengineering and Biopharmaceutical Research, Hanyang University, Seoul 133-791, South Korea ]
• Byung-Soo Kim [ School of Chemical and Biological Engineering, Seoul National University, South Korea ]
• DongYunLee [ Departments of Bioengineering, College of Engineering, and Institute for Bioengineering and Biopharmaceutical Research, Hanyang University, Seoul 133-791, South Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576