Earticle

초록

영어

The use of plant biomass for biofuel production will require efficient utilization of all sugars in cellulosic biomass, primarily glucose and xylose. While Saccharomyces cerevisiae can be engineered to ferment xylose, the engineered S. cerevisiae cannot utilize xylose until glucose is completely consumed. This sequential utilization of glucose and xylose results in lower yields and productivities of ethanol. To overcome this problem, we engineered S. cerevisiae to co-ferment cellobiose and xylose by introducing cellodextrin transporter (cdt-1) and intracellular β-glucosidase (gh1-1) from Neurospora crassa (1). The cellobiose fermenting S. cerevisiae was also able to co-ferment cellobiose and galactose with a higher ethanol yield and productivity, suggesting that the strain can be applied for utilizing marine biomass (2). Energetic benefits were improved through phosphorolysis pathway instead of hydrolysis pathway for cellobiose utilization (3). HXT2.4, the poor cellodextrin transporter from Scheffersomyces stipitis was drastically improved by substitutions of alanine (A291) to charged amino acids in HXT2.4 (4). These results suggest that efficient and rapid co-fermentation of cellobiose and other sugars can be achieved using the cellobiose-fermenting S. cerevisiae strains (5).

키워드

저자

• Suk-jin HA [ Department of Bioengineering and Technology, Kangwon National University. ]
• Soo Rin KIM [ Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign. ]
• Heejin KIM [ Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign. ]
• Eun Joong OH [ Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign. ]
• Myoung-Uoon JANG [ Department of Food Science and Technology, Chungbuk National University. ]
• Jonathan M. GALAZKA [ Department of Molecular and Cell Biology, University of California at Berkeley. ]
• Jamie H.D. CATE [ Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley. ]
• Yong-Su JIN [ Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576