This study will present efficient and environmental-friendly methods for the pretreatment of rice straw by using planetary or attrition mills. Both mill modes are effective to reduce size and cellulose crystallinity in rice straw. The relative crystallinity indexs were 0.20 and 0.69 for planetary milled and attrition milled samples. Enzymatic treatment of the milled rice straw produced both glucose and xylose. Total monosaccharides produced in enzymatic hydrolysis were 0.39 and 0.37 g/g rice straw for planetary and attrition mill treated, respectively. In comparison experiment to chemical pretreatments, none of the rice straw biomass was lost during the milling processes but it showed a 32.3 % and 10.3 % reduction in the SAA and NaOH pretreatments, respectively. Soaking the rice straw in both the aqueous ammonia and sodium hydroxide solutions led to the production of 0.95 and 2.34 g/l of freely soluble phenolic compounds, respectively. In contrast, the planetary and attrition mill pretreatments produced 3- to 9-fold less phenolics. To further investigate, the toxicity of each hydrolysate samples, a recombinant bacterium E. coli strain DH5α/pDMA3 was employed. The hydrolysates from the NaOH and SAA treatment elicited a 31-fold and 6-fold induction in bioluminescence while no induction was seen for both the planetary and attrition mills.
키워드
Rice strawBiofuelPretreatmentmilling
저자
Jin Hyung LEE [ Korea Institute of Ceramic Engineering and Technology, Seoul, 153-801. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동