Earticle

초록

영어

Cigarette smoking (SM) is considered to be well known environmental toxin which contributes to the onset of various diseases. SM cause direct lungs damage, activate lungs inflammatory responses, and in some cases leads to the development of lung cancer. Cytokines in injured starfish (Asterina pectinifera) is the potential changes in its expression during the regeneration process. Especially, expression of TGF-β1 has increased in arm cut starfish extract after eight days. Also, starfish including saponin like the ginseng. Saponin is widely used in the world because of some effective pharmacological activities. Therefore, the current study was designed to elucidate the pharmacological activities of starfish extract against cigarette smoking induced damage in cell line and pulmonary tissue. We investigate that the effect of eight days starfish extract after arm cut (8d) and intact starfish extract on cell line and mouse lung injury by SM. In cell proliferation analysis, although cigarette smoking extract (CSE) was co-treated, the higher proliferation ability is shown in 8d treatment than intact starfish extract. 8d and intact starfish extract was directly transported to pulmonary cells through respiratory organ by nebulizer inhalation. In this case of cigarette smoking, the pulmonary structure was damaged and functions become abnormal. However, 8d treated groups showed similar with the control group compared with SM group. Among them, 8d was proved to be more effective than intact starfish extract. These results demonstrate that 8d could more protect pulmonary structure and function than intact starfish extract against cigarette smoking by ginseng like saponin and regulation of inflammatory cytokines.

목차

Abstract
 1. 서론
 2. 재료 및 방법
  2.1. 시약 및 장비
  2.2. 재료 및 처리조건
  2.3. 불가사리 추출
  2.4. 담배연기 추출
  2.5. 세포주 및 실험조건
  2.6. 실험군 및 실험조건
  2.7. In Vitro 실험
  2.8. In Vivo 실험
  2.9. 폐의 채취 및 분리
  2.10. Hematoxylin & Eosin stain (H&E stain)
  2.11. 통계 처리
 3. 결과 및 고찰
  3.1. 세포 증식효과
  3.2. 세포내 단백질 활성 측정
  3.3. 체중의 변화
  3.4. Catalase 활성
  3.5. 지질과산화 측정
  3.6. 혈액성분의 변화
  3.7. 단백질 활성 측정
  3.8. 폐 조직의 변화
 4. 결론
 References

키워드

저자

• 황경희 [ Kyung Hee Hwang | 경북대학교 자연과학대학 생명과학부 ]
• 정혁 [ Hyuk Jung | 경북대학교 자연과학대학 생명과학부 ]
• 장수찬 [ Su Chan Chang | 경북대학교 자연과학대학 생명과학부 ]
• 박종석 [ Jong Seok Park | 경북대학교 자연과학대학 생명과학부 ]
• 김유영 [ You Young Kim | 경북대학교 자연과학대학 생명과학부 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576