Earticle

초록

영어

We investigated the anti-diabetic activity and enzymatic activity of 24 commercial doenjang samples certified for traditional foods. Twenty four doenjang samples showed the wide ranges in enzymatic activities (protease activities 0-50.45 unit/g, α-amylase activities 0-675.9 unit/g, β-amylase 13.6-308.6 unit/g), and there were no difference in enzymatic activity by the producing region. To evaluate the potential anti-diabetic activity of 24 doenjang samples, we examined the effect of doenjang methanol extract (DME) on 2-[n-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amyno]-2-deoxyd- glucose (2-NBDG) uptake. Ten samples among 24 samples significantly stimulated the uptake of 2-NBDG. When the cells were treated with DME at 400 ug/mL, No. 17 and 23 specially stimulated 2-NBDG uptake by 1.23-fold and 1.25-fold, respectively, compared with untreated control cell. And there were no cytotoxicity in the C2C12 cells treated with DME at concentration of 500 ug/mL. Among 24 samples, No. 6, 7, 12, 21 and 24 showed the α-glucosidase inhibitor activity at concentration of 10 mg/mL; however, they were less effective than acarbose which is a commercial α-glucosidase inhibitor.

목차

Abstract
 1. 서론
 2. 재료 및 방법
  2.1. 된장 시료의 수집
  2.2. 사용시약
  2.3. 조효소액 및 70% methanol 추출물 제조
  2.4. 효소활성 측정
  2.5. 2-NBDG uptake
  2.6. MTT
  2.7. α-glucosidase 저해활성 측정
  2.8. 통계처리
 3. 결과 및 고찰
  3.1. 효소활성 측정
  3.2. 2-NBDG uptake
  3.3. α-glucosidase 저해활성 측정
 4. 결론
 감사
 References

키워드

저자

• 이소영 [ So-Young Lee | 한국식품연구원 발효기능연구단 ]
• 김인선 [ In-Sun Kim | 한국식품연구원 발효기능연구단 ]
• 박소림 [ So-Lim Park | 한국식품연구원 발효기능연구단 ]
• 임성일 [ Seong-IL Lim | 한국식품연구원 발효기능연구단 ]
• 최혜선 [ Hye-Sun Choi | 농진청 국립농업과학원 농식품자원부 ]
• 최신양 [ Shin-Yang Choi | 한국식품연구원 발효기능연구단 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576