Byung-Joo Lee, Mi-Ae Lee, Yong-Gyun Kim, Kwang-Won Lee, Yong-Pyo Lim, Byung-Eui Lee, Ho-Yeon Song
언어
영어(ENG)
URL
https://www.earticle.net/Article/A194094
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4,000원
원문정보
초록
영어
Pleurotus ostreatus ‘Miso’ is a mutant strain showing white color in pileus from the known parent strain ‘Wonhyeong 1’. Shape and several other characters also vary with culture conditions. Mating experiments were performed to understand interstrain mating relationship using monokaryons of the parent and the mutant strains. All monokaryons were grown from single spores isolated from freshly collected fruit bodies. Pairings were performed in 90 mm petri dishes on PDA. They were allowed to grow at 25 until two fronts of the advancing mycelia met and developed a conspicuous contact zone. The contact zone and the outer edges of paired colonies on each plate were examined for clamp connections. The parent and the mutant resulted in tetrapolar incompatibility in intrastrain crosses. In interstrain crosses, each monokaryotic tester strain of the parent strain was out-crossed to monokaryotic tester strains of the mutant. As a result of these crosses it was found that both strains share the same A and B incompatibility factors yielding 25% compatibility.
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Conditions for fruiting bodies Single basidiospore isolates and monokaryons Mating tests Nuclear Staining RESULTS AND DISCUSSION REFERENCES