Earticle

현재 위치 Home

Poster session C : Medicinal Mushroom/Fungi

Induction of apoptosis by cordycepin in human leukemia U937 and THP-1 cells

첫 페이지 보기
  • 발행기관
    한국버섯학회 바로가기
  • 간행물
    한국버섯학회지 바로가기
  • 통권
    제8권 제4호 (2010.12)바로가기
  • 페이지
    pp.198-198
  • 저자
    Seong-Sook Park, Jin-Woo Jeong, Yung-Hyun Choi, Jae-Dong Lee
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A194023

원문정보

초록

영어
[Background] Cordyceps militaris is a traditional popular mushroom, produces an important bioactive compound Cordycepin (3’-deoxyadenosine) used for the tonic and medicinal purpose in eastern Asia. Cordycepin is reported to possess many pharmacological activities including immunologically stimulating, anti-tumor, anti-virus, and anti-infection effects. [Methods] Growth inhibition of human leukemia cells was assessed by MTT assays. The determination of apoptotic cell death was performed by flow cytometry analysis, agarose gel electrophoresis and DAPI fluorescent staining methods. The apoptotic-regulated gene markers in both death receptor- and mitochondria-mediated apoptotic pathways were detected by RT-PCR and Western blot analysis etc. [Results] It was found that inhibition of cell proliferation was observed for human leukemia U937 and THP-1 cells treated with cordycepin in a dose-dependent manner. Cordycepin induced morphological change and apoptotic cell death such as formation of apoptotic bodies, DNA fragmentation and increased populations of apoptotic-sub G1 phase. Apoptosis of U937 and THP-1 cells by cordycepin was associated with a down-regulation of anti-apoptotic Bcl-2 and inhibitor of apoptosis proteins (IAPs) expression. Cordycepin treatment induced the proteolytic activation of caspase-3, caspase-8 and caspase-9, and a concomitant inhibition of poly(ADP-ribose) polymerase (PARP), β-catenin and phospholipase (PLC)-γ1 protein. Conclusions: Our results indicated that the apoptotic processes caused by cordycepin are mediated by the regulation of the Bcl-2 and caspase family in human leukemia U937 and THP-1 cells. Our data also suggested that cordycepin may be a potential chemotherapeutic agent for the treatment of leukemia cancer patients.

저자

  • Seong-Sook Park [ Department of Microbiology, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea; ]
  • Jin-Woo Jeong [ Department of Microbiology, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea; ]
  • Yung-Hyun Choi [ Department of Biomaterial Control (BK21 program), Dongeui University Graduate School, Busan 614-714, Republic of Korea Department of Biochemistry and Research Institute of Oriental Medicine, Dongeui University College of Oriental Medicine, Busan 614-052, Republic of Korea ]
  • Jae-Dong Lee [ Department of Microbiology, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea; ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국버섯학회 [The Korean Society of Mushroom Science]
  • 설립연도
    1997
  • 분야
    농수해양>농학
  • 소개
    우리나라의 버섯 연구와 관련 산업의 발전에 기여하고 버섯관련 연구자와 관련산업 종사자간의 학술정보 등의 교류를 목적으로 한다.

간행물

  • 간행물명
    한국버섯학회지 [Journal of MUSHROOMS]
  • 간기
    계간
  • pISSN
    1738-0294
  • eISSN
    2288-8853
  • 수록기간
    2003~2025
  • 등재여부
    KCI 등재
  • 십진분류
    KDC 525 DDC 635

이 권호 내 다른 논문 / 한국버섯학회지 제8권 제4호

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.