Earticle

초록

영어

Four Ganoderma lucidum strains, Chizhi 05, Jingda, Huizhou and Xinzhou, were screened out as hybrid parent in order to establish G. lucidum cross breeding system that based on protoplast monokaryogenesis method. Monokaryotic strains of each parental strains were obtained and mating type of each monokaryotic strains were determined. One to three monokaryotic strains that have different mating types were mated, and hybrids were identified by clamp connection observation and antagonist response. The results showed that the number of monokaryon came from Chizhi 05, Jingda, Huizhou and Xinzhou was 9, 14, 40 and 38, respectively. Only one mating type was obtained from Jingda, and two mating types were obtained from the other three strains, Chizhi 05, Huizhou and Xinzhou, respectively. Chi-square test showed that the ratio of two mating types of the three strains was 1:1. Fourteen monokaryotic strains of different mating types from 4 parental strains were select as a cross- breeding materia, and 17 hybrids were obtained, which were identified by clamp connection observation and antagonist response. This study proclaimed that the practicality of the hybridization breeding of G. lucidum by protoplast monokaryogenesis method.

키워드

저자

• Wu Jiaojiao [ Northeast Agricultural University, Harbin 150030; Zhejiang Forestry Academy, Zhejiang Provincial Key Laboratory of Biological and Chemical Utilization of Forest Resource, Hangzhou 310023; ]
• Fu Lizhong [ Zhejiang Forestry Academy, Zhejiang Provincial Key Laboratory of Biological and Chemical Utilization of Forest Resource, Hangzhou 310023; Lishui Edible Fungi R & D Center，Zhejiang Essence Fungi Development Co., Ltd，Lishui 323000 ]
• Wu Xueqian [ Zhejiang Forestry Academy, Zhejiang Provincial Key Laboratory of Biological and Chemical Utilization of Forest Resource, Hangzhou 310023; Lishui Edible Fungi R & D Center，Zhejiang Essence Fungi Development Co., Ltd，Lishui 323000 ]
• Xu Xiuhong [ Northeast Agricultural University, Harbin 150030; ]
• Li Haibo [ Zhejiang Forestry Academy, Zhejiang Provincial Key Laboratory of Biological and Chemical Utilization of Forest Resource, Hangzhou 310023; ]
• Wu Qingqi [ Zhejiang Forestry Academy, Zhejiang Provincial Key Laboratory of Biological and Chemical Utilization of Forest Resource, Hangzhou 310023; Lishui Edible Fungi R & D Center，Zhejiang Essence Fungi Development Co., Ltd，Lishui 323000 ]
• Wei Hailong [ Zhejiang Forestry Academy, Zhejiang Provincial Key Laboratory of Biological and Chemical Utilization of Forest Resource, Hangzhou 310023; Lishui Edible Fungi R & D Center，Zhejiang Essence Fungi Development Co., Ltd，Lishui 323000 ]
• Cheng Junwen [ Zhejiang Forestry Academy, Zhejiang Provincial Key Laboratory of Biological and Chemical Utilization of Forest Resource, Hangzhou 310023; ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국버섯학회지 [Journal of MUSHROOMS]

• 간기

  계간

• pISSN

  1738-0294

• eISSN

  2288-8853

• 수록기간

  2003~2025

• 등재여부

  KCI 등재

• 십진분류

  KDC 525 DDC 635