Earticle

초록

영어

Effect of trehalose for the mycelial growth of Tricholoma matsutake were examined. When T. matsutake Z-1 strain was cultured in the partially modified matsutake liquid (PMML) medium and the Hamada matsutake liquid (HML) medium supplemented with trehalose at 24 ℃ for 80days, the vegetative mycelial dry weights showed higher value compared with those of PMML medium and HML medium supplemented with glucose (control). The range of the effect of 1.0-8.0% carbohydrate substrate on vegetative mycelial growth was investigated. The optimal concentration for mycelial growth was 2.0% for the glucose medium but 8.0% for the trehalose medium. To evaluate the potential of the production of trehalase from T. matsutake, the extracellular trehalase activity during the vegetative mycelial growth was measured. The activity of the extracellular trehalase increased during vegetative mycelial growth of T. matsutake and was maximal 70 days after inoculation. This extracellular enzyme was investigated for the purification and the characterization. The partially purified trehalase was obtained from about 1.53l static culture filtrate, with 19.1% recovery, and about 2,940 fold purification. The molecular mass was about 62.6kDa (SDS-PAGE) and 70kDa (Gel-filtration). The enzyme was most active around 40℃ and pH 5.0 and stable over a pH of 4.5~ 6.5 for 30min at 37℃. The enzyme readily hydrolyzed trehalose having α -1,1 glucosidic bond. However, it did not hydrolyze disaccharides such as maltose, iso-maltose, cellobiose, saccharose and lactose.

저자

• Mizuho Kusuda [ Laboratory of Biocycle Engineering, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen- cho, Naka-ku, Sakai, Osaka 599-8531, Japan ]
• Ryosuke Yodono [ Laboratory of Food Microbiological Science and Biotechnology, School of Agriculture, Kin-ki University, 3327-204 Nakamachi, Nara 631-8505, Japan ]
• Mitsuhiro Ueda [ Laboratory of Biocycle Engineering, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen- cho, Naka-ku, Sakai, Osaka 599-8531, Japan ]
• Norifumi Shirasaka [ Laboratory of Food Microbiological Science and Biotechnology, School of Agriculture, Kin-ki University, 3327-204 Nakamachi, Nara 631-8505, Japan ]
• Kazutaka Miyatake [ Laboratory of Biocycle Engineering, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen- cho, Naka-ku, Sakai, Osaka 599-8531, Japan ]
• Takao Terashita [ Laboratory of Food Microbiological Science and Biotechnology, School of Agriculture, Kin-ki University, 3327-204 Nakamachi, Nara 631-8505, Japan ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국버섯학회지 [Journal of MUSHROOMS]

• 간기

  계간

• pISSN

  1738-0294

• eISSN

  2288-8853

• 수록기간

  2003~2025

• 등재여부

  KCI 등재

• 십진분류

  KDC 525 DDC 635