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영어
Single ascospores was isolated in Yesan-gun, Chungnam in 2006 with Cordyceps militaris. This study was carried out to select the superior isolates which produce more fruiting body and contain more cordycepin contents. Five single ascospore isolates were cultured on brown rice pupae medium and they formed fruiting bodies. To select better isolates, each isolates was hybridized and then the characteristics and cordycepin contents were investigated. After biological efficiency and codycepin contents were examined, finally C0601S05 was selected. Top-cross test of isolate C0601S05 was carried with 29 single ascospores. From this test, isolate C0601S05×C0601S33 and isolate C0601S05×C0601S43 containing highest cordycepin contents were selected. Codycepin contents of C0601S05 was 246mg% and those of C0601S05×C0601S33 and C0601S05×C0601S43 were 325mg% and 280mg%, respectively. C0601S05×C0601S33 was registered as Yedang 3 at the Korea seed & variety service.
목차
ABSTRACT 서론 재료 및 방법 공시균주 코디세핀 정량분석 단포자 교배 및 자실체 특성검정 Top-cross test 결과 및 고찰 공시균주 단포자 교배 및 자실체 특성검정 Top-cross test 적요 감사의 말씀 참고문헌