Earticle

초록

영어

Agaricus blazei is well known as a traditional medicinal mushroom and it has been shown to exhibit immunostimulatory and anti-cancer activity. However, the cellular and molecular mechanism of apoptosis of cancer cells is poorly understood. In this study, we have investigated whether A. blazei extract (ABE) exerts anti-proliferative and apoptotic effects on human leukemia THP-1 cells. It was found that ABE induced a time- and dose-dependent increase in leukemia cells apoptosis through caspase-3 activation and PARP cleavage. Activation of caspase- 9 induced by ABE suggested that ABE-induced signaling was mediated through a mitochondrial death pathway. In addition, we observed an elevation of ROS and a consequent loss of mitochondrial membrane potential, further suggesting that ABE-induced death signaling was mediated through a mitochondrial oxygen stress pathway. The antioxidant Nacetylcysteine, however, opposed ABE-mediated mitochondrial dysfunction, caspase activation, and apoptosis, supporting the role of ROS in the apoptotic process. We conclude that ABE induces apoptosisin human leukemia cells through a reactive oxygen species and caspase-dependent mitochondrial pathway.

저자

• Mun-Ock Kim [ Department of microbiology, College of Natural Sciences, Pusan National University, Pusan, Korea ]
• Dong-Oh Moon [ Faculty of Applied Marine Science, Cheju National University, Jeju, Korea ]
• Jin-Woo Jeong [ Department of microbiology, College of Natural Sciences, Pusan National University, Pusan, Korea ]
• Cheng-Yun Jin [ Department of microbiology, College of Natural Sciences, Pusan National University, Pusan, Korea ]
• Gi-Young Kim [ Faculty of Applied Marine Science, Cheju National University, Jeju, Korea ]
• Jae-Dong Lee [ Department of microbiology, College of Natural Sciences, Pusan National University, Pusan, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국버섯학회지 [Journal of MUSHROOMS]

• 간기

  계간

• pISSN

  1738-0294

• eISSN

  2288-8853

• 수록기간

  2003~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 525 DDC 635