This study was carried out to obtain basic data on physiological characteristics for an artificial cultivation of fruiting body of Cordyceps. Specimens such as Cordyceps longissima, C. militaris and C. pruinosa were collected at Mt. Halla of Cheju island in July, 2003. Among four different culture media which have been used for culture of mushrooms, MCM medium was selected for the favorable culture medium of the Cordyceps tested. The initial pH of solid medium for mycelial growth of Cordyceps was good in the range of pH 5.0~7.0 lower than 8.0. The mycelial growth of C. longissima was most favorable on culture media supplemented with glucose, one of monosaccharides. In C. militaris, nine carbon sources were favorable to the mycelial growth as compared with control among 11 carbon sources. Six nitrogen sources were favorable to the mycelial growth of C. longissima as compared with control among 9 carbon sources; namely, the mycelial growth of C. longissima was most favorable on culture media contained potassium nitrate, and followed in order by ammonium citrate and sodium nitrate in 4 weeks incubation.
목차
ABSTRACT Materials and Methods Specimen Identification Isolates Inoculation Selection of optimal media Effect of pH on mycelial growth Effect of carbon source Effect of nitrogen source Results and Discussion Descriptions Cordyceps longissima Cordyceps militaris Cordyceps pruinosa Physiology Effect of pH Effect of carbon source Effect of nitrogen source References
In-Pyo Hong [ 홍인표 | Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, R.D.A., Suwon 441-707, Korea ]
Corresponding author
Sung-Hee Nam [ 남성희 | Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, R.D.A., Suwon 441-707, Korea ]
I-Yeon Jung [ Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, R.D.A., Suwon 441-707, Korea ]
Gyoo-Byung Sung [ 성규병 | Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, R.D.A., Suwon 441-707, Korea ]
Hack-Woo Nam [ 남학우 | Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, R.D.A., Suwon 441-707, Korea ]
Seok-Woo Kang [ 강석우 | Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, R.D.A., Suwon 441-707, Korea ]
Hyeon Hur [ 허현 | Dept. of Applied Biology, Dongguk University, Seoul 100-715 ]
Min-Woong Lee [ 이민웅 | Dept. of Applied Biology, Dongguk University, Seoul 100-715 ]
Shun-Xing Guo [ 곽순성 | Institute of Medicinal Plant, Chinese Academy of Medical Sciences, P.R.China ]