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Role of N-Acetylglucosaminyltransferase-V and galectin-3 binding protein in anoikis stress of cancer cells
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- 발행기관
- 한국당과학회 바로가기
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- 간행물
- 한국당과학회 학술대회 바로가기
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- 통권
- ACGG 2012 Conference (2012.10)바로가기
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- 페이지
- pp.111-112
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A192946
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원문정보
초록
- 영어
- Anoikis is an anchorage-dependent cell death in which an epithelial cell shows the apoptotic behaviors upon loss of adhesion with cells and extracellular matrix. Anoikis resistance is known to be a crucial step for cancer metastasis, and it is demanding that understanding of the mechanism underlying the process is required to control cancer metastasis at a molecular level. We have previously identified N- Acetylglucosaminyltransferase-V (GnT-V), a glycosyltransferase that catalyzes the addition of N- acetylglucosamine (GlcNAc) to the core moiety of N-glycan of glycoproteins, producing β1,6-linkages, is a key protein inducing metastasis in human colon cancer cells. And the secretion of galectin-3 binding protein (LGALS3BP) was lowered in GnT-V overexpressing WiDr cells. In this study, we hypothesized that GnT-V has an inhibitory effect on anoikis of cancer cells, while LGALS3BP stimulates the apoptotic process. To prove this, we established the stable transfectants (WiDr:GnT-V), the control cells (WiDr:mock). In addition, we investigated the molecular signatures in various colon cancer cell lines: WiDr, HCT116, SW480 and LoVo. To induce anoikis process, cells were cultured atop 1% soft agar-coated plate and their cellular and molecular responses were monitored. As a result, GnT-V was observed to mitigate anoikis and promote cell aggregation in soft- agar plate system. Among human colon cancer cells tested, HCT116 cells formed aggregates poorly and thus showed low cell viability. Of note, the secretion of LGALS3BP was higher than any other colon cancer cellline, which is in line with the result that anoikis resistance was observed in LGALS3BP-suppressed WiDr cells. Taken together, these results may suggest that GnT-V induces anoikis resistance of cancer cells either alone or in cooperation with the target molecules, such as LGALS3BP.
저자
간행물 정보
발행기관
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- 발행기관명
- 한국당과학회 [Korean Society for Glycoscience]
- 설립연도
- 2006
- 분야
- 의약학>약학
- 소개
- 본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.
간행물
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- 간행물명
- 한국당과학회 학술대회
- 간기
- 연간
- 수록기간
- 2006~2022
- 십진분류
- KDC 517 DDC 614
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