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Identification of a Tumor-Associated Autoantibody against Aminoimidazole Carboxamide Ribonucleotide Transformylase/Inosine Monophosphate Cyclohydrolase (ATIC) in Hepatocellular Carcinoma (HCC) Mouse Model and Its Application to Diagnosis of HCC

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    ACGG 2012 Conference (2012.10)바로가기
  • 페이지
    pp.111-111
  • 저자
    Chang-Kyu Heo, Mi-Kyung Woo, Hai-Min Hwang, Ah Ruem Kim, Dae-Yeul Yu, Ju Yeon Lee, Jong Shin Yoo, Chulhun Chang, Jeong Heon Ko, Eun-Wie Cho
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192945

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원문정보

초록

영어
Autoantibodies, which are generated by immune system recognizing the presence of the abnormal tumor-associated antigens, are promising biomarkers for the early detection of tumors. Recently, we established a B cell hybridoma pool derived from HBx transgenic mouse, as a source of tumor-associated autoantibodies without using any extracellular antigens, and have characterized the specific target antigens against them. XC154 autoantibody, one of them, has been investigated in this study and its target antigen was identified by mass spectrometric analysis as aminoimidazole carboxamide ribonucleotide transformylase / inosine monophosphate cyclohydrolase (ATIC) that catalyzes the last two steps of de novo purine biosynthesis. A specific mimotope against XC154 autoantibody was screened from the cyclic random hepta-peptide phage library and, using mimotope display M13 phage as a coating antigen for ELISA, we could distinguish patients with hepatocellular carcinoma (HCC) vs. normal subjects with 86.96% sensitivity and 88.24% specificity. These results imply that anti-ATIC autoantibody is induced in patients with HCC and detection of anti-ATIC autoantibody can be used for the diagnosis of HCC. Now, several attempts have been performed to establish a more reliable and convenient assay to detect tumor-assocoated autoantibodies using recombinant proteins displaying cycling peptide instead of mimotope display M13 phage and the usefulness of improved assay will be discussed.

저자

  • Chang-Kyu Heo [ Cancer Biomarkers Development Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]
  • Mi-Kyung Woo [ Cancer Biomarkers Development Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]
  • Hai-Min Hwang [ Cancer Biomarkers Development Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]
  • Ah Ruem Kim [ Cancer Biomarkers Development Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]
  • Dae-Yeul Yu [ Aging Research Center, KRIBB, Daejeon, Korea ]
  • Ju Yeon Lee [ Division of Proteome Research, Korea Basic Science Institute, Daejeon ]
  • Jong Shin Yoo [ Division of Proteome Research, Korea Basic Science Institute, Daejeon ]
  • Chulhun Chang [ Department of Laboratory Medicine, Pusan National University, Pusan, Korea ]
  • Jeong Heon Ko [ Cancer Biomarkers Development Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]
  • Eun-Wie Cho [ Cancer Biomarkers Development Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

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