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Aptamers against ManLAM of Mycobacterium tuberculosis inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeys

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    ACGG 2012 Conference (2012.10)바로가기
  • 페이지
    pp.84-84
  • 저자
    Qin Pan, Qilong Wang, Fengling Luo, Min Li, Xianru Xia Dongdong Shi, Xiao-Lian Zhang
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192902

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원문정보

초록

영어
Tuberculosis (TB) has been a major world-wide cause of death for centuries. One-third of the world’s population is infected with Mycobacterium tuberculosis (M.tb), the etiologic agent of TB. The development of potent new anti-TB drugs is urgently needed. The major M.tb surface lipoglycans, mannose-capped Lipoarabinomannan (ManLAM) had immunosuppressive effects during M.tb infection. In this study, aptamer ZXL1 which specifically bound to ManLAM from the virulent strain M.tb H37Rv was screened out by Systematic Evolution of Ligands by EXponential enrichment (SELEX). The binding affinity of ZXL1 to ManLAM was measured as 8.907X10-8 M of quilibrium dissociation constant (Kd) value by surface plasmon resonance (SPR) analysis. We found that aptamer ZXL1 prevented the ManLAM-induced immunosuppressive effects on DCs and inhibited M. tb entry into macrophages. More importantly, we found that single injection of aptamer ZXL1 significantly prolonged the survival rate of infected mice, and prevent the infected rhesus monkeys from weight loss. The Bacterial numbers were significantly lower in the lungs and spleens in ZXL1-treated groups. These results suggest that aptamer ZXL1 can be used as antimycobacterial agent or as TB vaccine adjuvent.

키워드

tuberculosis ManLAM aptamer

저자

  • Qin Pan [ State Key Laboratory of Virology, Department of Immunology, Hubei Province Key Laboratory of Allergy and Immunology,Wuhan University School of Medicine, Donghu Road 165#, Wuhan 430071, Hubei Province, China ]
  • Qilong Wang [ State Key Laboratory of Virology, Department of Immunology, Hubei Province Key Laboratory of Allergy and Immunology,Wuhan University School of Medicine, Donghu Road 165#, Wuhan 430071, Hubei Province, China ]
  • Fengling Luo [ State Key Laboratory of Virology, Department of Immunology, Hubei Province Key Laboratory of Allergy and Immunology,Wuhan University School of Medicine, Donghu Road 165#, Wuhan 430071, Hubei Province, China ]
  • Min Li [ State Key Laboratory of Virology, Department of Immunology, Hubei Province Key Laboratory of Allergy and Immunology,Wuhan University School of Medicine, Donghu Road 165#, Wuhan 430071, Hubei Province, China ]
  • Xianru Xia Dongdong Shi [ State Key Laboratory of Virology, Department of Immunology, Hubei Province Key Laboratory of Allergy and Immunology,Wuhan University School of Medicine, Donghu Road 165#, Wuhan 430071, Hubei Province, China ]
  • Xiao-Lian Zhang [ State Key Laboratory of Virology, Department of Immunology, Hubei Province Key Laboratory of Allergy and Immunology,Wuhan University School of Medicine, Donghu Road 165#, Wuhan 430071, Hubei Province, China ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

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