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Glycomics meets histopathology: Lectin microarray can profile the whole glycoproteins in specific groups of cells isolated by laser microdissection

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    ACGG 2012 Conference (2012.10)바로가기
  • 페이지
    pp.82-82
  • 저자
    Atsushi Kuno, Masaharu Nomura, Hideki Matsuzaki, Tomoko Nakagawa, Atsushi Matsuda, Yoshitoshi Hirao, Masao Sasaki, Norihiro Ikeda, Toshitaka Nagao, Yuzuru Ikehara, Hisashi Narimatsu
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192899

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원문정보

초록

영어
Cell glycome is defined by the glyco synthesis machinery regulated by harmonized expression of more than 100 glycogenes. The machinery-dependent glycome drastically shifts during cell progression and differentiation in association with tumorigenesis and malformation, and thus it motivates us to discover the disease-related alteration in glycosylation. Glycan-targeted histochemical approaches using lectin and anti-glycogene antibodies have provided some key information to characterize specific histological types of cells in pathology. However, this approach is not suitable for the comprehensive analysis targeting the cell glycome, and thus may fail to provide insight into glycome shift during the disease progression. Several years ago, we developed the methodology for rapid and systematic glycome shift analysis targeting formalin-fixed tissue specimens by means of lectin microarray. The resultant method enabled simultaneous observation of over 40 lectins interacted with glycoproteins in 1 mm2 of the tissue specimens. Recently, we sophisticated this methodology to be suitable for comparative analysis of a series of cells in specific groups isolated from a single tissue specimen by laser microdissection, and now our research has gained interest in the variability and distribution of cell glycome in the tissue, i.e., “tissue glycome mapping”. In this meeting, we will summarize the advantage of this new methodology and its application for glyco-biomarker discovery, as well as the construction of “tissue glycome atlas”.

저자

  • Atsushi Kuno [ Research Center for Medical Glycoscience (RCMG), National Institute of Advanced Industrial Science and Technology (AIST),Tsukuba, Japan. ]
  • Masaharu Nomura [ Department of Surgery I, and Diagnostic Pathology, Division, Tokyo Medical University Hospital, Tokyo, Japan. ]
  • Hideki Matsuzaki [ Research Center for Medical Glycoscience (RCMG), National Institute of Advanced Industrial Science and Technology (AIST),Tsukuba, Japan. ]
  • Tomoko Nakagawa [ Research Center for Medical Glycoscience (RCMG), National Institute of Advanced Industrial Science and Technology (AIST),Tsukuba, Japan. ]
  • Atsushi Matsuda [ Research Center for Medical Glycoscience (RCMG), National Institute of Advanced Industrial Science and Technology (AIST),Tsukuba, Japan. ]
  • Yoshitoshi Hirao [ Research Center for Medical Glycoscience (RCMG), National Institute of Advanced Industrial Science and Technology (AIST),Tsukuba, Japan. ]
  • Masao Sasaki [ Research Center for Medical Glycoscience (RCMG), National Institute of Advanced Industrial Science and Technology (AIST),Tsukuba, Japan. ]
  • Norihiro Ikeda [ Department of Surgery I ]
  • Toshitaka Nagao [ Diagnostic Pathology, Division, Tokyo Medical University Hospital, Tokyo, Japan. ]
  • Yuzuru Ikehara [ Research Center for Medical Glycoscience (RCMG), National Institute of Advanced Industrial Science and Technology (AIST),Tsukuba, Japan. ]
  • Hisashi Narimatsu [ Research Center for Medical Glycoscience (RCMG), National Institute of Advanced Industrial Science and Technology (AIST),Tsukuba, Japan. ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

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